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… A team has optimized a crucial part of CRISPR-Cas9 technology to enable improvements in microbial oil … Cas9 needs guide RNA to lead it to the correct sequence to snip — but not all guides are effective. To … approach: CRISPR-Cas9 relies on RNA with a short guiding sequence (20 bp) that directs the endonuclease where to cut, …

… CheckV can help researchers assess virus sequence fragments and data quality. The Science Through … and computational approaches, more and more virus sequences are being recovered and identified from … a previous effort, an international consortium recommended specific guidelines and best practices for characterizing …

… A workflow for capturing the genetic make-up of specific uncultivated microbes from complex environmental … The Impact Despite great strides in generating genome sequences for members of the microbial world through bulk … microbes within metagenome samples and associate their sequences with their roles within their environments. …

… a plant. The Science Sex in plants can be befuddling. Most species are hermaphrodites, expressing both male and female … first time, the sex chromosomes of shrub willow have been sequenced with sufficient resolution to analyze their … located at Lawrence Berkeley National Laboratory. The JGI sequenced the shrub willows through the  Community Science …

… development, dozens of reference genomes and thousands of resequenced sorghum varieties, a resource for breeders and … high-quality reference genomes. By cataloguing a genome sequence for a line of sorghum, reference genomes have … in lines, as well as how structural changes connect to specific traits. They also saw long-studied traits come into …

… yeast Saccharomyces cerevisiae , allowing this species to grow better than its closest relative S. … then used a series of random insertions of a foreign DNA sequence to generate a huge pool of mutants, each containing … died and others grew well; the survivors had their DNA sequenced at the JGI as part of a 2014 Community Science …

… and eukaryotic microbes and viruses in environmental sequence data. Dr. Schulz employs multi-omics (metagenomics, …