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From Intern to Published Scientist: The Journey of a JGI-UC Merced Intern
… a quest to better understand the factors and mechanics of gene expression in over 400 fungi species. “Fungi are the … “survival of the fittest” theory — the most expressed genes found across each of the fungal species were … protein-coding hotspots. “Ensuring that the most expressed genes are also the most efficient at making proteins likely …
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First Monoploid Reference Sequence of Sugarcane
… in these crops. For diploid species, sequencing programs generally focus on a genotype with two identical sets of … sequence using a targeted approach that focused on the gene rich part of the genome by harnessing information from … The reference sequence is useful for mapping the genes involved in sugar production and for identifying …
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Gut Fungi: Unexpected Source of Novel Chemicals
… which could inform how scientists can more easily convert plants into carbon-neutral biofuels . What’s more, many … Swift — verified that the fungi expressed biosynthetic gene clusters, which are “building blocks” of natural … products. The team then confirmed that these expressed genes translated into expected proteins. That work was done …
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JGI@25: Strengthening Soybean
… of Missouri said he and his colleagues had identified 1,110 genes involved in lipid metabolism.  “There’s still a lot of … progress had already been made positionally cloning genes involved in agronomic traits related to nutritional … “All along the way, it was just like rediscovering genetics, with the evolution of sequencing technologies and …
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Getting to the Bottom of Fungal Functions Across Earth’s Forests
… project to form new connections between fungal guilds, genes and function. If a tree falls in the forest — whether … in four forest ecosystems, extracted RNA to understand gene expression, and developed new tools to map that soil … and nutrients to trees; plant pathogens colonize living plants to feed on them.  Across the forest types, Martin and …
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A More Intuitive Phytozome Interface
… for over 5 years, provides users with the genome browsers, gene pages, search, BLAST and BioMart data warehouse … Phytozome v12, and that most bookmarked v12 links (e.g., to gene reports and organism information pages) will be … https://phytozome-next.jgi.doe.gov/info/Ptrichocarpa_v3_0 . Gene pages will also be forwarded (e.g.,  Gene …
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Better Genome Editing for Bioenergy
… The Science CRISPR-Cas9 is a powerful, high-throughput gene-editing tool that can help scientists engineer … to snip — but not all guides are effective. To ensure the gene editing tool will make the right cut, researchers … of guide RNAs that were effective against 94 percent of the genes in a lipid-prolific yeast. The Impact The team used …
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JGI@25: From Life at the Extremes to Editing Genomes
… refine and improve the process of finding novel Cas genes and CRISPR systems. The collaboration has resulted in … Combining forces with UC Berkeley to seek elusive gene-editing proteins … NERSC & JGI Seek Elusive Proteins for Gene Editing …
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Sequencing Sphagnum Leads to Discovery of Sex Chromosomes
… of the world’s soil carbon. The process to harvest energy-generating peat from these boggy wetlands produces an … type of microbes that break down plant matter. This means plants don’t fully decompose in peatlands, and so carbon … was theorized that sex determination was possibly driven by genetics, or even temperature. By sequencing the genome, …
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Building Wet and Dry Lab Skills
… project is to determine how mutations in plant transporter genes affect microbiome composition and plant growth. Under … steps of the experiment from growing and inoculating the plants to harvesting the plants and extracting DNA. They also analyzed 16s amplicon …
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JGI Part of Berkeley Lab Team Awarded Best Use of HPC in Life Sciences
… DOE Joint Genome Institute (JGI), and the Energy Sciences Network (ESnet) have been recognized for outstanding … genomics in support of DOE missions related to clean energy generation and environmental characterization and cleanup. …
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Applying Viral Genomics Expertise to Understanding the Human Virome
… between viruses and their host microbes impact the regulation of global nutrient cycles. The JGI’s latest … viruses are likely to infect, and (iii) identifying the genetic traits of the viruses to better understand their … , 2021), geNomad , a tool for identifying mobile genetic elements ( Nature Biotechnology , 2024), and iPHoP , …
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