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2024 Progress Report
… use of our data. We completed 39,870 projects in FY24 We generated 871 Terabases of sequence We synthesized 9.7 Megabases of DNA We processed … We have also scaled DNA-affinity purification sequencing (DAP-seq),  stable isotope probing (SIP) and omics, and we …
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Micro-Scale Applications Group
… The Micro-Scale Applications group explores the genetic makeup of natural microbial communities using a … functional assay lets us move beyond cataloging the genes of microorganisms and start making quantitative … access to cutting-edge cell sorting, DNA amplification, and sequence analysis to recover the genomes of uncultivated …
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An Integrated Dataset to Understand Ecosystem Interaction
… value as both biofuel feedstock and their role in carbon sequestration. Poplar trees grow quickly in a variety of … the trees, microbes and soil chemistry offers insights into how these ecosystems cycle nutrients like nitrogen and … surroundings — illuminating the molecular mechanisms for adaptation within the trees. The data set generated is 25 …
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Mapping the Earth’s Hidden Fungal Kingdom
… to Antarctic valleys, these organisms have diversified into an estimated 2–6 million species — dwarfing the … about 200,000 fungal species have been named. Among those sequenced, roughly half of all genes have completely unknown … meaning hidden within millions of genes? Fostering a Deeper Understanding Fortunately, fungi are ideal subjects for …
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Community Science Program
… community at large with access to high-throughput sequencing, synthesis, and metabolomics at the JGI for … call is for projects that translate genomic information into biological function. The  New Investigator and … based on their DOE relevance.  For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …
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IMG/VR
… Roux describes the structure and content of the IMG/VR v3 database (img.jgi.doe.gov/vr), the latest release of IMG … to browse and search through the more than 2 million viral sequences available in IMG/VR v3. …
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How Filamentous Fungi Sense Food
… from simple to complex carbohydrates, and then convert them into simple sugars. More importantly, when faced with a … simple sugars to plant biomass. One of these techniques was DAP-seq, a high-throughput method for identifying protein … We acknowledge the use of deletion strains generated by Grant P01 GM-068087 “Functional Analysis of a …
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Innovative Technology Improves Our Understanding of Bacterial Cell Signaling
… changes in response to the molecule of interest. The team adapted a previously developed combination CSL-BRET approach … Ec YcgR, within the Nano-lantern scaffold. They then mined sequence databases for homologous YcgR proteins and chose a … were synthesized as codon optimized sequences and cloned into the Nano-lantern scaffold. Because the biosensors are …
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Programs & Platform Leads
… to support BER's mission of achieving a predictive understanding of complex biological, earth, and …
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Denoising inferred functional association networks obtained by gene fusion analysis
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CalTeach
… for undergraduate majors interested in developing a deep understanding of STEM teaching & learning. Their coursework …
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JGI Plant Gene Atlas: an updateable transcriptome resource to improve functional gene descriptions across the plant kingdom
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