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… to only three phyla. The Woyke group uses single-cell sequencing as a means to begin filling this gap. A primary … of interest, prior to and in conjunction with whole genome sequencing. … B., Woyke T ., Ivanova N. N., Kelly W. J. Cultivation and sequencing of rumen microbiome members from the Hungate1000 …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary … you have no idea how to put it back together. So a lot of sequencing depends on Illumina technology, which is usually …

… samples in from afar, without ever hearing much about the sequencing lab. So today, Chris Daum walks through the JGI’s sequencing pipeline, where there are freezers with names — …

… and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … We have a lot more data. Obviously, there’s been a lot of sequencing going on, especially at the JGI and in other … very excited to learn more and hear more about what JGI and collaborators are discovering. DAN: Alright, well, I’m glad …

… Bill Fenical. And we connected to JGI through the Community Sequencing Program, and they worked with us to sequence two … at Monterey Bay, to be able to then do, at real time, sequencing on the underwater rover while it’s in the middle … Dan: So these are going to be seaweed metagenomes? You’re sequencing everything? Brad: Metagenomes and genomes. We’re …

… the Hawaiian bobtail squid. And so more recently one of my collaborators Spencer Nyholm has been working on the … really exciting work on figuring out how to get genomic sequencing data for the fungus garden because it’s more … in this project, the interaction with JGI was really around sequencing for, I think, for the fungus garden and some of …

… timeline of sorghum projects at the JGI: 2009: JGI and collaborators use whole-genome shotgun sequencing to analyze the 730-megabase Sorghum bicolor (L.) … it with over 50 other diverse sorghum genomes. Deep sequencing and genetic linkage analysis improve the genome’s …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … in-house is really important because even with the best collaborators, there’s always a lag when you’re trying to … to understand more about plant natural products? We have sequencing facilities, DNA synthesis, metabolomics. Where do …

… everything is funding, right, isn’t it? I thank JGI for sequencing a lot of fungi. But the thing with them – to get … sequence is really cheap. I’ve got colleagues that are sequencing dozens and dozens of fungi, mostly coming out of … course, are much smaller. But I think a lot of groups are sequencing fungi. Just going and sequencing them. Yeah.   …

… next generation of genomic research users, partners, and collaborators.  Ms. Ballon is a molecular biology-trained …