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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… With a new methodology, scientists are now getting more complete genomic results from single cells. The … method called Primary Template-Directed Amplification (PTA) yields a much higher completion, above 80% in most cases. … it’s difficult to link the connections to them from the genetic elements that scientists have found, e.g. plasmids …
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The Power of One, Amplified
… A preview of how large-scale single cell genomics complements metagenomics studies. The … that species specific sets of single cells harbored mobile genetic elements that were missed within paired metagenome … of within species recombination, indicating variation in gene flow between the analyzed community members. The Impact …
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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… A group led by JGI scientists have made strides in improving and applying a method that allows researchers … domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, … can deliver multiple types of payloads, such as individual genes, whole pathways, genome-editing tools, and reporters.” …
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Benjamin Cole
… Dr. Cole is interested in studying how plants interact with their … their growth program, and on how plants coordinate their gene expression with light and temperature oscillations … how microbes can colonize plant roots, focusing on genetic components required for effective colonization. In …
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How Maize Makes An Antibiotic Cocktail
… Maize produces a family of compounds that protects it against infections — using surprisingly few enzymes. The … buck. The research team reports that transferring just four genes encoding these enzymes to another plant, such as  … They screened panels of hundreds of maize lines for genetic variation in the ability make zealexins, and …
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Graduate Students Get Thesis Research Opportunity at the JGI
… Kaze, part of the 2018 cohort of the JGI-UC Merced Genomics Internship Program. Kaze is part of Mark Sistrom’s lab at … a remarkable opportunity to launch a pilot project and begin the work of understanding how engineered environments … between the two institutions to help train the next generation workforce.  Since its inception in 2014, more …
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Bacteria and Fungi Divvy Up the Work in Forest Floor
… second fiddle to those from fungi when it comes to breaking down tough plant fibers. The Science Walk in a temperate … teems beneath your feet. Both bacteria and fungi have genes that can break down plant fibers. But are they both … sequences (transcriptomes) from their soil samples. This gene expression dataset was particularly important for …
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A Plantiful Future: Xiaohan Yang
… Can plants help humans attain a renewable energy future? Can they help lock away more … Laboratory, believes they can. And, what’s more, that using gene editing technology to conscientiously mix traits of …
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JGI announces 2023 CSP Functional Genomics awardees
… research from potential users who need help translating genomic information into biological function. Through our CSP … and omics capabilities to enhance understanding of gene and genome function.  Below find a list of the six …
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JGI announces final round of 2022 Functional Genomics awardees
… research from potential users who need help translating genomic information into biological function. Through our CSP … and omics capabilities to enhance understanding of gene and genome function.  Below find a list of the final …
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Climate Change Threatens Base of Polar Oceans’ Bountiful Food Webs
… warm-adapted microbes edge polewards, they’d oust resident tiny algae. The cold polar oceans give rise to some of the … microbiomes that influence algal diversity and gene expression. Sailing from pole to pole on four research … algal communities on filters, they sequenced DNA ‘marker’ gene sequences to identify the microbes. And in order to …
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JGI Workshop at SIMB NP Meeting
… and their biosynthetic logics to be understood, accessing these untapped metabolic capabilities is often tedious and resource intensive. This is often due to, for example, tight regulation of biosynthetic gene clusters (BGCs) in native hosts, the complexity of …
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