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Mapping N-Linked Glycosylation Sites in the Secretome and Whole Cells of Aspergillus niger Using Hydrazide Chemistry and Mass Spectrometry
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Frequently Asked Questions: Project Management
… ) Metagenomes and Single Cells: IMG/M   (citation: … for functional annotation/curation of your genomes Microbial (IMG–ER) Metagenome and Single Cell (IMG/ M-ER) …
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Danielle Goudeau
This work combines custom flow cytometric analysis, cell sorting, genome amplification, PCR screening, and 16S … She also develops novel flow cytometry protocols for targeted recovery of specific microbes. …
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New Lineages of Life Group
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Viral Genomics Group
… parameters driving virus:host dynamics in environmental microbial communities. This involves both experimental and …
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Tracking the Origins of Methane-Producing Microbes
… recent studies now suggest the gene cluster predates this microbial group. Recently published in the Proceedings of … researchers applied mini-metagenomics to these samples by sorting the microbes into small pools of cells that were then sequenced separately. From these …
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The Power of One, Amplified
… single cell genomics by collecting nearly 500 single cells from a single low diversity hot spring sediment … of bioinformatics tools for  extracting snippets of microbial genomes directly from environmental sequence data, … used as a complement to isolate sequencing, i.e. when the targeted cells cannot be cultivated in the lab. What makes …
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JGI Early Career Researchers Featured in mSystems Special Issue
Metagenome Program head Emiley Eloe-Fadrosh outline more targeted approaches to reconstruct individual microbes in an … metagenomics. One approach uses fluorescence-activated cell sorting (FACS) to sort cells into several small pools before … sequence reads to link mobile elements such as plasmids to microbial host cells. In a separate article, research …
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Rex Malmstrom
… high throughput approach for isolating individual bacterial cells and amplifying their genetic material, thus bypassing … that reveals in situ functional activities of specific microbial groups within complex communities.  The Microscale … including those that require fluorescence activated cell sorting and non-standard molecular protocols.  Finally, his …
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Natural Prodcast Episode 12: Nadine Ziemert
… to get into first, and the standing trees. And he had this whole idea of NaPDoS , this computer program which uses … So I think I spent two years only looking at pathways the whole day. No, yeah, and then I think that having all these … work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger sequencing, and really …
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Natural Prodcast Episode 21: Elizabeth Parkinson
… month, just like I promised. Now I just have to make this whole “on-time thing” a habit! I’m really not good at that, … outreach program to get kids interested in microbiology and microbial chemistry. All good stuff, and I hope you enjoy … was saying, grad school is difficult. And that just add a whole other level of complexity and-- BETSY PARKINSON: I …
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Fishing for Novel Cellulose Degraders
… characteristics. When they tested the screen method on a microbial community from geothermal hot springs, they … particles through the “hook” of fluorescence-activated cell sorting to sort cells into several small pools, followed by whole genome …
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