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… With a new methodology, scientists are now getting more complete genomic results from single cells. The … years, extracting single cells and then amplifying their DNA for genome sequencing was no easy task. The traditional … cell breaks apart and its DNA is copied millions of times using a method named Multiple Displacement Amplification to …

… A group led by JGI scientists have made strides in improving and applying a method that allows researchers … CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the … for our users,” says JGI Director Nigel Mouncey. Using CRAGE, JGI has established a portfolio of microbial …

… part of CRISPR-Cas9 technology to enable improvements in microbial oil production. The Science CRISPR-Cas9 is a … sgRNA sequences: six for every gene in the genome. The JGI  DNA Synthesis Science team synthesized these sgRNAs, cloning … to be required for growth. By contrast, an initial screen using an unvetted library (with both active and inactive …

… High-throughput stable isotope probing helps ID novel interactions between … isotope to it. The isotopes are incorporated into the DNA of specific microbes who consume the labeled food or … The work conducted by the U.S. Department of Energy Joint Genome Institute ( https://ror.org/04xm1d337 ), a DOE …

… Machine learning approach significantly expands inovirus … which are filamentous viruses with small, single-stranded DNA genomes and a unique chronic infection cycle. “We’re not … microbial genomes and 2,249 of the 6,412 (35 percent) metagenomes mined for this study. “We’re simply getting much …

… An improved genome assembly to support targeted tree breeding for the bioeconomy.   The Science Researchers report a … to provide an improved eucalyptus genome assembly generated using advanced technologies to reconstruct the complete … Africa. The South African team collected the plant tissues. DNA and RNA sequencing were conducted by the JGI, along with …

… A former JGI intern is now applying his internship lessons on presentations to peatlands … fine tune his approach. “I got a lot of that from Zhong.”   Publication: Carrell AA et al. Habitat-adapted microbial …