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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… ( CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the genome. “CRAGE is central to our ability to offer host-engineering for our users,” says JGI Director … domesticate some 60 strains, inserting into them a genetic sequence that acts as a “landing pad.” Mouncey adds, “Once …
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JGI Hosts GPU Hackathon in May
Computing Facility (OLCF), NVIDIA, and the Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science user facility, aims to explore applications in …
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Boosting Small Molecule Production in Super “Soup”
… still work in cell-free environments? (Spoiler: Yes.) Think of cells as tiny factories: within their walls they have … , researchers led by Hal Alper at The University of Texas at Austin and Michael Jewett of Northwestern … and cleanup. The JGI provides integrated high-throughput sequencing and computational analysis that enable …
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Researching and Solving Real-World Problems with the 2023 JGI-UC Merced Interns
Emerald Nash and mentor Jonelle Basso, Experimental Study of Root Colonization by Resident Phages of a Plant Growth … mentor Bryce Foster, Benchmarking Bioinformatics Tools for Sequence Similarity Searching Morgan Lavenstein Bendall and … And we’ve been working on isolating and cultivating and sequencing extremophile protists from a hypersaline alkaline …
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Tracking the Origins of Methane-Producing Microbes
… archaea are estimated to produce 500 million tons of methane a year, which is over half the total global … the microbes into small pools of cells that were then sequenced separately. From these samples, 111 … and cleanup. The JGI provides integrated high-throughput sequencing and computational analysis that enable …
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Access to Rock Star Scientists
… their work delivered during the 5th anniversary celebration of the internship program. Candace Cole, UC Merced JGI Mentors : Ben Cole & Axel Visel, Genomics of Plant-Microbial Interactions “The summer internship has further confirmed by desire to pursue a STEM career, specifically in a government lab. My favorite thing is the …
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A One-Stop Shop for Analyzing Algal Genomes
… data portal allows anyone to freely investigate the genes of more than 100 algae. The Science Interested in the … algal genomes can be incredibly complex and difficult to sequence and understand. A team of researchers led by Algal … inquiry: it shows a full phylogenetic tree of more than 100 sequenced algal genomes — many sequenced by the JGI — so …
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A One-Stop Shop for Analyzing Algal Genomes
… data portal allows anyone to freely investigate the genes of more than 100 algae. The Science Interested in the … algal genomes can be incredibly complex and difficult to sequence and understand. A team of researchers led by Algal … inquiry: it shows a full phylogenetic tree of more than 100 sequenced algal genomes — many sequenced by the JGI — so …
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Tanja Woyke Elected to American Academy of Microbiology
Tanja Woyke , who has been elected to the American Academy of Microbiology (AAM) ! She is among the 68 new Fellows in the Class of 2020, selected from 118 nominees. The selection process … One example, published in Nature , applied single-cell sequencing to decode genomes of bacteria and archaea from …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… from single cells. The Science Studying the  genomes of microorganisms is incredibly useful for understanding the … usually employ two different methods; shotgun metagenomic sequencing and single-cell sequencing. For years, extracting single cells and then …
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Engagement Webinar: What Can You Do With An Approved CSP Functional Genomics Call Proposal?
… really fully exist back then,” said Hal Alper, a University of Texas at Austin professor, “but we really first heard … how different bioenergy relevant plants can produce species-specific blends of terpenoids, naturally occurring organic … the researchers will receive a FedEx package with the sequenced, quality controlled population representing the …
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Engagement Webinar: How to Successfully Apply for a CSP Proposal
… and relevance to the DOE’s mission.  Have someone proofread your proposal to make sure the goals, experimental … in their proposal as long as they’re staying under the sequence cap? A(nswer): Proposals that are looking at … multiple ways to can mix and match synthesis, metabolomics, sequencing. The only requirements are that you stay under …
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