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JGI@25: Expanding Metagenomics to Capture Viral Diversity
… In honor of the JGI's 25th anniversary in 2022, we have revisited a number of notable achievements … conditions and a specific microbial host to replicate. Sequencing genetic material directly from samples sidesteps …
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Streamlining Regulon Identification in Bacteria
… RIViT-seq technology could speed up associating transcription factors with their target genes. The … of regulon identification by in vitro transcription-sequencing (RIViT-seq) to facilitate the characterization of …
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From Berkeley to Binghamton: Tracking Strawberry Evolution
… In the summer of 2015, Adam Session was a postdoc working at … and the  oilseed crop false flax – genomes that have been sequenced by the JGI – and the common strawberry, which is … clearly identifies the four subgenomes based on non-genic sequences.”  In his words, the history of the strawberry is …
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Innovative Technology Improves Our Understanding of Bacterial Cell Signaling
… Newly developed chemiluminescent biosensors shed light on how bacteria function and … Ec YcgR, within the Nano-lantern scaffold. They then mined sequence databases for homologous YcgR proteins and chose a … These 92 homologues were synthesized as codon optimized sequences and cloned into the Nano-lantern scaffold. Because …
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JGI@25: Studying Sorghum’s Survival Skills
… In honor of the JGI's 25th anniversary in 2022, we have … but other important crops as well. In 2009, the JGI sequenced and analyzed the first reference genome for … JGI: 2009: JGI and collaborators use whole-genome shotgun sequencing to analyze the 730-megabase Sorghum bicolor (L.) …
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A Pinch of Salt Activates Silent Gene Clusters in Streptomyces
… This single environmental trigger highlights efficiency for users … salt triggers this metabolic shift, the team combined RNA sequencing across multiple time points with Cappable-seq, a technique that precisely maps where transcription … This work conducted by the U.S. Department of Energy Joint Genome Institute (https://ror.org/04xm1d337), a DOE …
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Forming Scientific Connections and Building Community
… The 11th cohort of JGI-UC Merced interns pick highlights from their summer experiences. This year the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science User … Nandita Nath & W. Berkeley Kauffman Project: “ 16s Sequencing of Barcoded Microbial Genomes ” Morgan Lavenstein …
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Eelgrass Proves to Be Much Younger Than We Thought
… The Science Eelgrass, one of the most abundant plants in the ocean, originated in Japan before spreading around … meadows are responsible for almost one-third of carbon sequestered in the ocean. In addition to providing a more … locations.  One group investigated changes in protein sequences, while others analyzed single point mutations …
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Characterizing Communities: JGI Announces Latest CSP Portfolio
… Proposals aim to sequence and annotate genomes from Antarctica to Africa to … Science Program of the U.S. Department of Energy (DOE) Joint Genome Institute (JGI), a DOE Office of Science user … redistributing water availability. By sequencing the millet rhizosphere and endosphere communities both with and without …
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Taking Stock of the Known and Unknown Microbial Space
… diversity hotspots Over the past few decades, advancements in sequencing technologies have led to an abundance of … genomic biodiversity. Using publicly available genome sequence data generated over the past three decades, their … (BER) missions. The JGI provides integrated high-throughput sequencing and computational analysis that enable …
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Learning to Look
… Machine learning approach significantly expands inovirus … first worked on a reference dataset that included genome sequences known to be affiliated with the Inoviridae. “What … features that you can use to identify the inoviruses.” Overhauling the Perception of Inovirus Diversity The results …
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An Inside Look at How Plants and Mycorrhizal Fungi Cooperate
… A new view into a symbiosis could offer benefits to address climate … to study both sides of this interaction up close, using RNA sequencing to understand gene expression: one of the first … or other biological activity of the surrounding neighborhood,” said Benjamin Cole, senior author of this work. Cole …
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