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Identification of candidate genes in Arabidopsis and Populus cell wall biosynthesis using text-mining, co-expression network analysis and comparative genomics
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Decomposing a San Francisco estuary microbiome using long-read metagenomics reveals species- and strain-level dominance from picoeukaryotes to viruses
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but most species do … years, extracting single cells and then amplifying their DNA for genome sequencing was no easy task. The traditional … cell breaks apart and its DNA is copied millions of times using a method named Multiple Displacement Amplification to …
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Plotting a Model for Virus-Host Warfare Deep Below Ground
…   Visualizing a novel, candidate viral genome found in the deep subsurface ecosystem. The Science Altiarchaea are … attempts at virus infections. Their genomes include short DNA sequences that code for CRISPR systems, which help … populations respond to virus infections, researchers are using Altiarchaea as a model system to study virus-host …
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An Integrated Dataset to Understand Ecosystem Interaction
… Research yields a comprehensive tree and soil dataset for understanding the dynamic relationship between poplar trees … wildlife habitats and water-quality regulation. Focusing on the rhizosphere and interactions between the trees, … metagenomics, scientists were able to analyze the DNA of microbes living around the tree roots in the …
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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… A group led by JGI scientists have made strides in improving and applying a method that allows … CRAGE ). CRAGE enables scientists to insert large pieces of DNA (up to 60 kb) in a single step, directly into the … for our users,” says JGI Director Nigel Mouncey. Using CRAGE, JGI has established a portfolio of microbial …
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Enlarging Windows into Understanding Gene Functions
… of these four letters represents an organism’s genetic code, or genome, which underlies physical features and … factors that control how genes are turned on is known as DNA affinity purification sequencing or DAP-seq. The … TFs and regulatory networks are conserved across fungi.” Using multiDAP-seq, Nagy’s team is comparing five fungal …
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Supercharging SIP in the Fungal Hyphosphere
… method to the study of a particular fungi, researchers identified novel interactions between bacteria and the fungi. … Many microorganisms hold clues to processes like biodegradation, with potential applications in industry and … isotope to it. The isotopes are incorporated into the DNA of specific microbes who consume the labeled food or …
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The ectomycorrhizal fungus Paxillus involutus converts organic matter in plant litter using a trimmed brown‐rot mechanism involving Fenton chemistry
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New and Improved: Eucalyptus Reference is Now Accessible
… 2014 Eucalyptus grandis reference genome that has been widely adopted. The new genome assembly resolves both … to provide an improved eucalyptus genome assembly generated using advanced technologies to reconstruct the complete … Africa. The South African team collected the plant tissues. DNA and RNA sequencing were conducted by the JGI, along with …
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Rex Malmstrom
… Dr. Malmstrom is a Staff Scientist and leader of the Microscale Applications group. Before joining the JGI in 2010, he made the first ever measurements of in situ growth rates and … work at the Massachusetts Institute of Technology he developed a high throughput approach for isolating …
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SPRUCE-ing Up Science
… Originally from northern California and one of the first students to participate in the DOE Joint Genome Institute … sink environments can continue to hold onto their carbon despite global warming.  At ORNL, Lawrence brings a … fine tune his approach. “I got a lot of that from Zhong.”   Publication: Carrell AA et al. Habitat-adapted microbial …
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