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… embargo starting at construct or strain delivery.  Detailed sequence information for constructs are made publicly …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what caves …

… (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other … annotation, comparative analysis, and interpretation of sequence, metabolomics, and functional genomics data types …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We … An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria Check out the agenda for the JGI’s 2021 Annual …

We focus our efforts on integrating database mining and sequence design with scaling the production of large, … developing methods for converting digital information from sequence databases into biotechnological or environmental …

… 11, 2022, in Cell Genomics , the team focused on Actinobacteria ( Actinomycetota ), a group of bacteria with … Group. Many of these bacterial and archaeal genome sequences have been generated using techniques that the JGI … Over the years more than 3,400 genomes have been sequenced from curated DSMZ isolates, for which quality DNA …

… through end-to-end automation, and further advancing sequence applications, single cell genomics, DNA synthesis, …

… tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et … al. (2015) Microbial species delineation using whole genome sequences. Nucleic Acids Res. 43(14):6761-71 Kyrpides NC et …

… particularly sigma factors that initiate transcription in bacteria. Using this technology, which combines an in vitro … expand the known transcriptional regulatory network in bacteria, enabling the discovery of regulatory cascades as … the 5’-ends by 5’-end sequencing, from which those promoter sequences recognized by a given sigma factor are inferred. …

Bacterial enzymes play second fiddle to those from fungi … So, to be able to identify proteins, the team compiled DNA sequences (metagenomes) and messenger RNA sequences (transcriptomes) from their soil samples. This …