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… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … in there. Or they can also have a lot of micro algae just living on the surface: diatoms, dinoflagelates, which can …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … where the microbiome of a plant, so the bacteria living on and inside the roots are protecting the plants …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … been described in such detail. DAN UDWARY: Alison, feel free to jump in with questions. But otherwise I’m going to … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… and environmental sustainability. Fungi, with million species, are essential for ecosystem functioning — … The  1000 Fungal Genomes Project is a five-year project to sequence 1000 fungal genomes from across the Fungal Tree of … of fungi. Mycocosm enables users to navigate across sequenced fungal genomes, and to conduct comparative and …

… through isolation and genome sequencing of new fungal species.  Students collect fungi, sterilize tissue, isolate … course materials and bioinformatics exercises are freely available through the links below. Naturalist Myco Ed … and distribution of fungal genome datasets. It includes JGI-sequenced and publicly available datasets encompassing a …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … at the micro organisms on those particles, as well as like free-living you know, within the Vibrio genus. So, when I’m… …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… embargo starting at construct or strain delivery.  Detailed sequence information and constructs are made publicly …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… recognize this material, though my show is a little more free-form, and this is the longer cut of the discussions you … at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …