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… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … favorite examples of a secondary metabolite producer, and why they produce those metabolites? MARC: Well, I’m really …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … are capable of making those strange ring systems. JACKIE: Why? Is it just because of ring strain or is it-- I guess, …

… We focus our efforts on integrating database mining and sequence design with scaling the production of large, … developing methods for converting digital information from sequence databases into biotechnological or environmental …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … biology perspective, like if you have a cluster of genes, why can’t you just engineer you know, a promoter or an …

… I request a culture/slant/DNA of an organism that has been sequenced at the JGI? …

… comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes …

… Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the … bit about your perspective on that kind of technique and why it’s valuable to you? Kate Duncan: I think both genome …

… tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other …

… the foundation for a large-scale CSP proposal submission. Sequence requests should not exceed 3 Tb in total. Proposals …