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… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … So, one of those is Acinetibacter . This is a genus of bacteria that’s really popped up at funding agencies and the …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. Not a …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… years, has been to bring generalizable technologies to bacteria, yeasts and algae previously with little to no …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … a check-up on me. And I wonder how that intersects with bacterial bioprospecting that is often done, where we’re … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… in plants, fungi, microalgae, environmental viruses, and bacteria to contribute to a more sustainable world. Learn …

… embargo starting at construct or strain delivery.  Detailed sequence information for constructs are made publicly …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what caves …

… (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other … annotation, comparative analysis, and interpretation of sequence, metabolomics, and functional genomics data types …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … section. We want to be sure that the PIs can articulate why their work falls within one of these DOE mission areas. …

… to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We … An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria Check out the agenda for the JGI’s 2021 Annual …