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… already had a fermentation process with already engineered strains, but this was to further build on those to try and … and so my team, we were responsible for coming up with new strains and new process improvements and new ways to recover … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the delineation of host-virus … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et … of Bacteria and Archaea: Sequencing a Myriad of Type Strains. PLoS Biology  12(8):e1001920 Ivanova N. et al. …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? … It almost killed me when I was three. It wasn’t an MRSA strain at the time because that was in the 1980s. I’m …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … get made fun of by my now-wife, because she was wondering why it is that we needed to produce more toxins in the lab …

… We focus our efforts on integrating database mining and sequence design with scaling the production of large, … developing methods for converting digital information from sequence databases into biotechnological or environmental …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … favorite examples of a secondary metabolite producer, and why they produce those metabolites? MARC: Well, I’m really …

… I request a culture/slant/DNA of an organism that has been sequenced at the JGI? …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … this and that. They’re doing all this 16S and I said, “Why don’t you just include the ITS too? It’s so easy to add …

… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …