Search

… and track record of the applicant. For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …

… an unlimited number of proposals).  For accepted proposals, sequencing, synthesis, metabolomics and analysis will be …

… which was a very early genome mining company, back when DNA sequencing of natural product pathways was hard and … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … it was a big deal. And you really had– I mean, the whole sequencing endeavor, had a cost, how much blood, sweat, and …

… that will be subject to the DOE JGI’s capabilities for sequencing, synthesis and/or metabolomics. Once materials … issues that may arise. You may monitor the status of your sequencing project at any time by logging on to the Genome Portals using your JGI login. After sequencing/metabolomics has completed, your Project Manager …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … technology.] BETSY PARKINSON: OK. DNA affinity purification sequencing. And so the way this essentially works is you … at the college level. So-- DAN: Yeah, for sure. And JGI is sequencing quite a few of the things that end up-- BETSY …

… and the 90s. And so we started to be able to explore DNA sequencing and actually get to the DNA sequence – sequences … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … We have a lot more data. Obviously, there’s been a lot of sequencing going on, especially at the JGI and in other …

… your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … JGI and this was before they were integrated. So I thought, why not feed it in there. And I went to group meeting and … AARON PURI: Yeah. No, you’re exactly right. So you can see why these are tightly regulated. DAN: For sure. For sure. …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … about his move to microbiology, about drug discovery and why it’s so hard to succeed, and we talk about an ongoing … bring it into the lab. And I think that criterion explains why the natural products in the ocean weren’t studied up …

… methods for efficient QA/QC based on next-generation sequencing technologies including PacBio and Illumina.  The …

… manager may then schedule and allocate resources for DNA sequencing. … No. However, the JGI does maintain a list of … … Guidance on international shipments can be found in the Sequencing Sample Overview file in our HERE (TK) .  Note: … appropriate alternatives. See specific protocols within the Sequencing Sample Overview file in our Documents Center, and …

NIGEL MOUNCEY: Oh, sure. I think our legacy obviously is in sequencing.  DAN: Of course.  NIGEL MOUNCEY: Sequencing the wazoo out of anything that’s relevant to the … so it’s really functional genomics. And there’s a lot of sequencing based approaches, of course, now that we can use …