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… before– or kind of around about when the first genome sequencing was done. But natural products– marine natural … that. Dan Udwary: Yeah, making biofuels has to be really cost-efficient or you have to extract some other value from … products chemistry. I get asked a lot about modern or mixed approaches, and we use a lot of those in my group. But, …

… really the same kind of thing. Before the age of genome sequencing, we had to basically break up the pieces of DNA … And you would scan those pieces by hybridization or maybe sequencing if you were rich enough. [CHUCKLES] And once you … Yeah, it certainly could. You can imagine lots of different approaches to sort of tackling this kind of a problem that …

… deciphering regulatory networks, and refining experimental approaches to explore the synthesis and function of these … We have developed Regulon Identification by in vitro-sequencing (RIViT-seq) to identify RNA polymerase-sigma … We are also utilizing DNA-Affinity Purification (DAP) sequencing to express hundreds of transcription factors and …

… [project] with JGI, how many flavin monooxygenases are you sequencing, sort of along that vein, of if you have one or … guess with along that line, with JGI, like how many are you sequencing currently? ALISON NARAYAN: Yeah. So– yeah, with …

… things, because a lot of what we do at JGI in terms of the sequencing that we do is very people-disconnected, and … of what you do then? And are there ways to do data-driven approaches at some point like that in the future? CASSANDRA … to understand more about plant natural products? We have sequencing facilities, DNA synthesis, metabolomics. Where do …

… the Broad that was looking to develop new methods for DNA sequencing of microbes. And what kept breaking our methods … So you were saying, Mark, how the DNA that was breaking the sequencing from really being functional was these secondary … what’s exciting and what’s not? MARC: Yeah, so one of the approaches that Tiny Earth is using to be different than …

… are microbes like bacteria, not humans. With metagenomic sequencing, researchers have found more of these viruses … viruses can do. Building on existing virus-host prediction approaches, researchers have created a new program called … learning model in the iPHoP program creates a way of evaluating these different routes. To train the model, the …

… include providing technical input on large-scale production sequencing, new sequencing technologies, and related informatics; overview …

… about the metabolomic data that, very nicely, with new approaches like that one push forward by Pieter Dorrestein – … this approach. DAN UDWARY: It’s really great. The other approaches of using homology, you’re only going to find the … us, we want to hear from you. We have projects in genome sequencing, DNA synthesis, transcriptomics, metabolomics, …

I think. DAN UDWARY: Yeah, and that was a JGI Community Sequencing Project. You’re right, yeah. NADINE ZIEMERT: Yes. … UDWARY: I think that was one of the JGI’s early moves into sequencing things for the purpose of looking at the … And we sequenced the whole fosmid at the time with Sanger sequencing, and really going from the ends and sequencing, …

Bill Fenical. And we connected to JGI through the Community Sequencing Program, and they worked with us to sequence two … at Monterey Bay, to be able to then do, at real time, sequencing on the underwater rover while it’s in the middle … Dan: So these are going to be seaweed metagenomes? You’re sequencing everything? Brad: Metagenomes and genomes. We’re …