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Recent Activity in Expanding Populations and Purifying Selection Have Shaped Transposable Element Landscapes across Natural Accessions of the Mediterranean Grass Brachypodium distachyon
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Heterologous Expression and CRISPR/Cas9-Assisted Manipulation of the Hybrid Gene Cluster Specifying the Biosynthesis of Meroterpenoids and Phenazines
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Danielle Goudeau
… cell sorting, genome amplification, PCR screening, and 16S sequencing. She also develops novel flow cytometry protocols for targeted recovery of specific microbes. …
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Extracting the Secrets of Secondary Metabolites
… survival, may now be easier to characterize following a proof-of-concept study in which researchers paired CRISPR and … Short Palindromic Repeats) is the leading tool for accurately editing genomes, its effectiveness has … Biology . When it comes to insect killer P. luminescens specifically, understanding its secondary metabolites and …
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High Throughput Phosphoglycolate Phosphatase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader
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Mining Metagenomes for Cas Proteins
… Institute.  The Science Researchers report the discovery of miniature Clustered Regularly Interspaced Short … Institute (JGI), a DOE Office of Science User Facility. The sequences were then biochemically characterized by a team … Institute. The Impact The ability to accurately edit genomes (that is, repair gene mutations, and …
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JGI@25: Using Team Science to Build Communities Around Data
… In honor of the JGI's 25th anniversary in 2022, we have revisited a … Spatafora of Oregon State University led the effort to sequence 1000 fungal genomes to create two reference genomes … genomes that are both large and complex. The first publication of a genome generated by the OGG initiative — …
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Nigel Mouncey
… the fourth Director in its 27-year history. He has a wealth of managerial experience from almost 20 years in the … focused on the discovery, development and commercialization of novel production organisms and fermentation processes for … on secondary metabolites. He is also on the Leadership Team of the National Microbiome Data Collaborative and served as …
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Biochemical characterization of Fsa16295Glu from “Fervidibacter sacchari,” the first hyperthermophilic GH50 with β-1,3-endoglucanase activity and founding member of the subfamily GH50_3
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Webinar: SIP technologies at EMSL and JGI
… Joint Genome Institute (JGI) provide access to a variety of tools that leverage stable isotopes to measure carbon and … go from start to finish, i.e., QC to separation to library sequencing and assembly. Like others, our turnaround time … the question might also hinge on if you’re interested in a specific metabolite. If you could do some sort of targeted …
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User Programs
… For accepted proposals, sequencing, synthesis, metabolomics and analysis will be paid for by the Department of Energy’s funding of the JGI. There is no cost to the user … with DOE mission-relevant projects that call for expansive sequencing, synthetic biology, and metabolomics capabilities …
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The Busy World of Deep Sea Eruptions
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