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An Age of CRAGE: Advances in Rapidly Engineering Non-model Bacteria
… in improving and applying a method that allows researchers to quickly modify diverse bacterial genomes. In 2019, the … be engineered. “Piloting access to this emerging technology with JGI users will enable us to assess CRAGE’s impact, … such as individual genes, whole pathways, genome-editing tools, and reporters.” JGI-developed genetic engineering …
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Exploring Possibilities: 2022 JGI-UC Merced Interns
… in science. Hear more about what they learned from working with their JGI mentors, what challenges they overcame, and what new skill sets … And I’m excited to take that with me, along with other tools that I can use to, um, perform computational biology.  …
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Understanding Wildfire Recovery, Starting in Soil
… a wildfire burns, recovery begins at the forest floor. Within the soil, a microbiome of bacteria, viruses and fungi … and nitrogen, paving the way for future plants and trees to grow. However, fire changes the microbes within the soil. … that can promote tree establishment and growth.  This study builds on the pyrophilous fungi the JGI has previously …
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The More the Merrier: Making the Case for Plant Pan-genomes
… plants abide by the concept, “the more the merrier,” with respect to their genomes. In their base state, they are diploids … B. hybridum  genomes and many  B. distachyon genomes. This builds upon a previously made  B. distachyon pan-genome. A …
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JGI@25 RECAP: 2022 Annual Meeting features historical perspectives on JGI’s 25 years
… featured speakers who shared a look back at the JGI’s history, from its beginnings in the sequencing for the Human … Nigel Sussman recently finished at the Integrative Genomics Building, the JGI’s home at Berkeley Lab. Like the mural, … into his role as program manager in 2006 when the JGI transitioned from human to microbial genetics. Drell held …
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Fishing for Novel Cellulose Degraders
A “bait and hook” single-cell genomic approach to bioprospecting. The Science One of the most vital pieces of equipment for fly fishing is a boxful of lures. Designed with feathers or wires to mimic an insect or a particular …
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2024 Progress Report
The Integrative Genomics Building (IGB) seen above is home to the DOE Joint Genome … processed 13,700 metabolomics samples With our users, 247 publications were published   … Our primary objective is to …
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JGI@25: The Human Genome Project, or the JGI’s Origin Story
… that showcase our collaborations and capabilities to enable great science that will help solve energy and … in 1990 when the U.S. Department of Energy (DOE) partnered with the National Institutes of Health. The DOE works with … with the HudsonAlpha Institute for Biotechnology. Publications Grimwood, J., Gordon, L., Olsen, A. et al. The …
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Calculating the Costs of Multiple Switchgrass Gene Copies
Scientists have discovered that the candidate bioenergy feedstock switchgrass has adapted to expand its habitat range. … also hoped to use this unique opportunity to quantify how transitions to higher ploidy could be generating these … climate resilient switchgrass for bioenergy production. Building on the framework provided by our new switchgrass …
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JGI@25: From Life at the Extremes to Editing Genomes
… that showcase our collaborations and capabilities to enable great science that will help solve energy and … as having “a revolutionary impact on the life sciences.” Building upon CRISPR-Cas9 work, the Doudna lab at UC … nature was using it to do something important.” Related Publications:  A scoutRNA Is Required for Some Type V …
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Better Genome Editing for Bioenergy
… team has optimized a crucial part of CRISPR-Cas9 technology to enable improvements in microbial oil production. The … of researchers sought to disrupt every gene in the genome with CRISPR-Cas9 in order to interrogate each one’s … were reliable. In an elegant, quantitative screen, the team designed a pool of sgRNA sequences: six for every gene in …
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JGI@25: Studying Sorghum’s Survival Skills
… that showcase our collaborations and capabilities to enable great science that will help solve energy and … biofuels thanks to its drought and flood hardiness. Even with little water, full sunlight and hot temperatures, … reference genome for Sorghum bicolor . Since then, 2,993 publications, 107 patent applications, and 53 patents have …
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