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… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … what we’re trying to do right now is develop the chassis strains that then we can port things into as well. DAN: Give …

… algae, examining both natural populations and engineered strains. This approach allows for the identification of key … The  1000 Fungal Genomes Project is a five-year project to sequence 1000 fungal genomes from across the Fungal Tree of … of fungi. Mycocosm enables users to navigate across sequenced fungal genomes, and to conduct comparative and …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … people went as far as saying, OK, we’re going to take 300 strains from our labs and measure them on their mass spec …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a … and you might have the same cluster and all these different strains. But only one produces the compound. So I think …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … certain clusters are more amenable to work with, or even strains. And I think then it lends more to microbiology. And …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … or low-nutrient. And over a six-month period, we found 20 strains that became visible. We took off the plates. We … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … OK. Oh, awesome. DAN: So yeah. If you get any cool strains you need some help, we'll probably be able to help …

… feedstock into valuable bioproducts, but many  Clostridium strains are difficult to engineer. Yasuo Yoshikuni and his …

Program aims to generate large datasets to explore sequence-function relationships, advancing the discovery of … Science Group supports researchers exploring how to convert sequence into functional assessments. The team has a … of fungi. Mycocosm enables users to navigate across sequenced fungal genomes, and to conduct comparative and …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …