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… legal counsel representative). The User Agreement and corresponding Appendix must be signed and returned before work on … be notified by your project manager in the initial correspondence with you if the User Agreement and Appendix are … dry/lyophilized pellet. It is usually very difficult to resuspend the pellet once it is received.  If you have concerns …

… JACLYN WINTER: Dropping F bombs. We should do some kind of special sound effect like a kazoo. DAN UDWARY: I do have … is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… running in your family. Dan: So tell me about that. What sparked it? I mean, what made you want to work in natural … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …

… aflatoxin being a secondary metabolite from an Aspergillus species that is pretty toxic – can cause liver … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

… that are known to be sources of secondary metabolites, like sponges were a really common source for people to try to … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… viral ‘dark matter’:   Viruses are abundant in the biosphere and influence microbial dynamics, evolution, and … comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes … Prospective users with questions on how to partner with the …

… and Appendix. An Appendix identifies the type of work or specific proposal to be covered under the Agreement. The … in a central office, such as President, Rector, CEO, Sponsored Projects Officer/Director or Legal Representative. … The lead institution will be required to assume responsibility for all material transfers. *University of …

… and this will be episode 11. This continues our little sprint on genome mining. In the last episode , Alison and I … about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… software includes: gRNA-SeqRET , an organism-agnostic CRISPR gRNA design tool BOOST , which provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… the right to produce manuscripts for publication and the responsibility for leading the efforts to do so, and JGI expects acknowledgment (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other … provide Users with a list of contributors and their corresponding ORCiD and relevant CRediT taxonomy categories. …

… good description of anyone because chemistry means a lot, especially when you’re working in natural products right. … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … Those are very– those are deserts. They’re very, very sparse areas. And we have certainly– when we first started …