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… there. And then, you know, they will have to create an environment that’s good for them to grow in. They might have … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … --really high. DAN: Yeah. Graduate school is a pressured environment, right? JACKIE: Yes. DAN: And so there's always-- …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … are marine related, and so we can do some projects that are freshwater and coastal. But we’ve kind of shied away from …

… And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … see what caves have to offer because they’re unique environments. These are structures that are millions of years …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … on synthetic biology of engineering gene expression in bacteria – however, the professor was actually quite … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less … in the lab, we’re not growing them in their natural environment, we’re not subjecting them to the environmental …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … unique they are, how they survive in this hypersaline environment, which can range from 8% to 28% salinity. We have … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… presentation from the 2019 JGI Genomics of Energy & Environment Meeting.] He’s also a really good friend of mine. I … at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … of the most notorious toxins there are in the marine environment. And it was that year that the Pacific Northwest …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … school children and understanding the plants and their environment. We also were able to build a botanical garden, … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … Illinois’ Institute for Sustainability, Energy, and Environment (iSEE) and the Carl R. Woese Institute for Genomic … also has a major focus on sustainability, examining the environmental and socioeconomic implications of moving to a …