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… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … really what my research focus has been. Maybe the last four or five years or so, this involves, you know, how …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … out the quorum sensing signals very quickly. So there’s four carbons that are derived from methionine, you run two …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … one of the most common animals in the ocean. They’re really species-diverse. I lost track actually of the number. But, … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… embargo starting at construct or strain delivery.  Detailed sequence information and constructs are made publicly …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… (see Auspice Statement below) for the generation of sequence or metabolomic data, DNA synthesized, and any other …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… shown today is it's more a quorum sensing within a single species. DAN: Within a species. Genus? BETSY PARKINSON: So … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… so I have an undergraduate in chemistry. Now when I was in fourth year, we had a choice to do an integrated master’s … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a … are novel. I mean, we have Streptomyces , but they’re new species. And they have very similar [genomes] to other …