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… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … on synthetic biology of engineering gene expression in bacteria – however, the professor was actually quite … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … So, one of those is Acinetibacter . This is a genus of bacteria that’s really popped up at funding agencies and the …

… cell types and associated organisms, including fungal and bacterial symbionts, detrimental microbial pathogens, and … This is a dynamic and complex ecosystem that includes bacteria, fungi, viruses. Resident viruses can be found in a myriad of bacterial strains, whereby they can modulate host …

Laboratory. Being a User Facility means that scientists from all over the world bring us big problems related to … chemical compounds that you find in plants, fungi and bacteria, basically all living things. When your crazy Aunt … stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … Initially, my group really focused on marine actinobacteria. Then we moved into marine proteobacteria. Not a …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what caves …

QC Protocol DNA Extraction 101 : tips, kits, and protocols (from PacBio) Guidance for sample collection and storage (from PacBio) Best practices for High Molecular Weight DNA … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… is focused on the discovery and characterization of novel bacterial, archaeal and eukaryotic microbes and viruses in environmental sequence data. We use multi-omics (metagenomics, … 1-9 Schulz et al. (2017) Towards a balanced view of the bacterial tree of life. Microbiome. 5 (1), 1-6 Schulz et al. …

… culture-disconnected. Like we’ll take some soil samples and sequence all the bugs out of that or whatever. And so I was … a check-up on me. And I wonder how that intersects with bacterial bioprospecting that is often done, where we’re … methicillin-resistant Staphylococcus aureus . Can you share why finding treatments for these microbes is so important? …

… in the analysis and publication of the claimed genome sequences. This opportunity, provided by the Department of … … A primary purpose of this project is to bring educators from a broad range of disciplines up-to-speed with some of … sequence to biological inference. … Genome sequences of key bacterial symbionts of entomopathogenic nematodes: …

The ranked proposal list, along with a recommendation from management at JGI and the other participating … … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… in Nature and Medicine.” It’s obviously focused more on the bacterial side of the field, and it’s a little hard to find … able to explore DNA sequencing and actually get to the DNA sequence – sequences for the genes that code for the … Something that’s you know …  ALISON: Central! DAN: That’s why they called it that! But for, maybe, people who are less …