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… of it. So it wasn’t only chemistry or only biochemistry or genetics. It was also ecology. It was also evolution. It was … that example, was it the case where that colleague saw a potential mechanism for why there would be this … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … It was unpublished at that point. But people would push out gene clusters maybe once a month at most. Maybe twice a … not been previously evaluated. So we started to see this potential of looking for compounds based on these gene …

… oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain … this really, really interesting. Is there an opportunity potentially for them to come work in the lab and actually do …

… Our research focuses on the assembly of metagenomic sequence data into microbial genomes, which is of … ecology and metabolism by elucidating the functional potential of hard-to-culture microorganisms. This process enables our users to explore the genetic makeup of microbial communities, shedding light on …

… and the science and scientists of secondary metabolism. Welcome back for our second season of Natural Prodcast. … more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … ALISON TAKEMURA: I know that it’s looking in the genome for genetic elements of interest, you know, something like …

… the active site of an enzyme. So there is no promiscuity, generally speaking, in primary metabolism. Nevertheless, … other compounds that are produced, and we don’t know what potentially other targets, if there are actually other … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… Science One of the most vital pieces of equipment for fly fishing is a boxful of lures. Designed with feathers or … of microbial enzymes, which could have a plethora of potential applications to industrial processes. Summary JGI … cell sorting to sort cells into several small pools, followed by whole genome amplification and …

… technology to help access complicated natural product gene clusters, and we’re all really excited about that, and … and enabling technology, but interesting biology and a potential application of translation in medicine. So it’s a … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… my undergraduate in microbiology and then PhD in microbial genetics in the UK. And came across to the US for a couple … products work, right? Think about how many things that are potential drug molecules that have micromolar activities. … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and 200 polar/500 nonpolar metabolomics samples, and should generally conform to the guidelines for our  CSP Large Scale … are strongly encouraged to contact the JGI to determine general feasibility prior to submitting a BER proposal that …

… association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … part of that, the monooxygenases, has to do with the mechanism. And so these enzymes use O2, and one of those oxygen … could have arisen from. DAN UDWARY: OK, so sort of a phylogenetic reconstruction kind of a thing. ALISON NARAYAN: …

… Program supports a wide range of projects, from single-gene synthesis to the synthesis of entire chromosomes, … the program enables the design and synthesis of complex genetic pathways and biosystems. It The program fosters … Program aims to generate large datasets to explore sequence-function relationships, advancing the discovery of …