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A programmable droplet-based microfluidic device applied to multiparameter analysis of single microbes and microbial communities
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Leo Baumgart
… his doctoral research in synthetic biology and microfluidics at the University of California, San Diego. As the Sequencing Technology Implementation Lead, he directs a … team responsible for piloting and operationalizing new genomic technologies, integrating automated liquid handling …
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Nonhybrid, finished microbial genome assemblies from long-read SMRT sequencing data
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Katherine Louie
… where she manages metabolomics production for community sequencing projects and heads R&D efforts to expand the metabolomics capabilities and product offerings for JGI users.  Using cutting-edge mass … and custom software for integrating metabolomics with genomic data (MAGI) for functional annotation and …
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Engagement Webinar: Harnessing JGI’s Metabolomics Capabilities
… (CSP) . This includes mass spectrometry-based measurements of primary and secondary metabolites from plants, … analyses? A:  For the JGI  New Investigator and  Functional Genomics proposal calls, projects can request up to 50 polar … The JGI is not a funding organization. Rather, it makes its sequencing, synthesis, metabolomics and data analyses …
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Generation of Transcript Assemblies and Identification of Single Nucleotide Polymorphisms from Seven Lowland and Upland Cultivars of Switchgrass
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Open Frameworks Training the Next Generation of Scientists
… technologies provide opportunities for students and professors alike The Science In  PLoS Pathogens , a team of … higher education that develops skills in fungal biology and genomics.  Myco-Ed :  The Mycological Curriculum for … provides training and scientific contributions—including sequencing services and analysis—to participants. Over the …
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Understanding Wildfire Recovery, Starting in Soil
… A metagenomic look at the soil microbes present a year after a … begins at the forest floor. Within the soil, a microbiome of bacteria, viruses and fungi process carbon and nitrogen, … a step further, using both DNA and messenger RNA (mRNA) sequencing to identify those traits that have helped fungi, …
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JGI Early Career Researchers Featured in mSystems Special Issue
… In a special issue of mSystems , out May 14, 2019, JGI researchers are among … beyond the tried-and-true method of traditional shotgun metagenomics. One approach uses fluorescence-activated cell … (FACS) to sort cells into several small pools before sequencing to recover rare microbes. The technique was …
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Tracking the Origins of Methane-Producing Microbes
… Mini-Metagenomics Approach Helps Identify Novel Archaeal Methane … archaea are estimated to produce 500 million tons of methane a year, which is over half the total global … more with JGI Microbial Program head Tanja Woyke and her Single Cells team member Frederik Schulz to screen the …
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New Research Sheds Light on Diversity in the Deep Sea
… get any sunlight, and yet they still support a unique array of microorganisms adapted to living in extreme conditions. … at Lawrence Berkeley National Laboratory, contributed metagenomic sequencing to the study as well as  genome analysis, … Science Program award 339, lead Peter Girguis) for sequencing several of the samples. …
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Gene Expression: From On-Off Switch to Dial
… to expression, scientists can now opt for various shades of grey. The ability to fine-tune the level of gene … the production of betaxanthins. They used combinations of single guide RNA, or a single RNA piece that recognizes a … area in the DNA, to determine which targets might benefit from an intermediate level of expression. Similar to the …
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