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How to Target a Microbial Needle within a Community Haystack
… A workflow for capturing the genetic make-up of specific uncultivated microbes … The Impact Despite great strides in generating genome sequences for members of the microbial world through bulk community sequencing (metagenomics) and single-cell genomics, the vast majority of …
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Nikos Kyrpides
… Dr. Kyrpides joined the DOE Joint Genome Institute in 2004 to lead the Genome Biology … for microbial genomes and metagenomes (IMG). He became the Metagenomics Program head in 2010 and has the combined … BA in Biology, Aristotle University of Thessaloniki, Greece PhD …
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Soil virus offers insight into maintaining microorganisms
… impact on the microbial communities within soil. Using metagenomics, scientists can shed light on hidden … AMGs in soil viruses thought to break down chitin, the gene sequences were cloned by the JGI.  The JGI’s DNA synthesis … and AMGs. It could spawn future research into understanding why these AMGs exist in the first place, and what function …
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JGI@25: Using Team Science to Build Communities Around Data
… In honor of the JGI's 25th anniversary in 2022, we have … Spatafora of Oregon State University led the effort to sequence 1000 fungal genomes to create two reference genomes … for an encyclopedia of reference genomes for fungal metagenomics. Two more CSP proposals came in 2016 that would …
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DOE JGI Metagenome Workflow
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Terabase-scale metagenome coassembly with MetaHipMer
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In Hot Spring Microbial Mat, Viruses Ride “Piggyback”
… a team has comprehensively described host-virus dynamics in a microbial mat. The Science In microbial mats, … National Laboratory, used single-cell sequencing to sequence both a cell’s genome and detect accompanying viral … the virus in 34 hosts or 26 percent of the single cells. Metagenomics sequencing then allowed the team to ask, for …
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Mapping the Earth’s Hidden Fungal Kingdom
… Leveraging massive comparative genomics to decode millions of … about 200,000 fungal species have been named. Among those sequenced, roughly half of all genes have completely unknown … for prediction of fungal lifestyles based on genome sequences.” The Fungal Knowledge Gap The fungal kingdom …
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JGI-USCCN Strain Sequencing Opportunity
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Metagenomic and Metatranscriptomic Analyses Revealed Uncultured Bacteroidales Populations as the Dominant Proteolytic Amino Acid Degraders in Anaerobic Digesters
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JGIota: The Algae Nicknamed ‘Chlamy’
… This shorter episode is about a tiny, single-celled alga – Chlamydomonas reinhardtii – that’s … UC Berkeley plant biologist Sabeeha Merchant explains why she works on this alga, how researchers managed to sequence its genome, and what it has to teach us about other …
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Pinning Down a Piece of Photosynthesis
… By studying the structure and function of a cyanobacterial protein, … of that machine — photosynthesizers’ DNA and RNA. These sequences provide a list of machine parts, and an idea of … the cell, Grosjean used the JGI-developed  guideRNA and Sequence Extraction Tool (gRNA-SeqRET) to produce knock-out …
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