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So I would say we run enzymatic reactions anywhere from 4 degrees to room temperature, to slightly elevated. We … So we’ll run things in like little Eppendorf tubes, or in 96, or 384 well plates. But then the fun really starts when … reconstruction”,  PNAS.  2023,  120, e2218248120. doi : 10.1073/pnas.2218248120 …

Anomaly” first described by Stanley and Konopa in 1985, which highlights our skewed understanding of microbial … through global metagenomics.” Nature 578(7795): 432-436 (2020). Seshadri R., Leahy S. C., Attwood G. T., Teh … chemoautotrophic symbiont genome. Science 315, 998-1000 (2007). Woyke T ., Teeling H., Ivanova N.N., Huntemann M., …

… 10 to the negative 6th. Nanomolar, 10 to the negative 9th. Picomolar, 10 to the negative 12th. DAN: Yeah. So … And so we're working with the JGI to actually explore 92 of these receptors both from clusters with known and … nearby. And of those once we did the predictions, about 140 unique cyclic peptides. And from that, about 130 of them …

S, Otani H, Udwary DW, Doering DT, Mouncey NJ. Cytochromes P450 involved in bacterial RiPP biosyntheses. J Ind Microbiol … of the genus Streptomyces. Sci Rep. 2022 Nov 7;12(1):18909. Otani H, Mouncey NJ. RIViT-seq enables systematic … through a novel mechanism. Nucleic Acids Res. 44, 595-607. Otani, H., Lee, Y.E., Casabon, I., and Eltis, L.D. …

… for a SIP-focused workshop at the JGI in the fall of 2024. “ We wanted to get into the nitty gritty with the people …

… the Joint Genome Institute.  … FY20 Progress Report … FY19 Progress Report … FY18 Progress Report … FY17 Progress … Report … FY16 Progress Report … FY15 Progress Report … FY14 Progress Report … FY13 Progress Report … FY12 Progress … Report … FY09 Progress Report … FY08 Progress Report … FY07 Progress Report … FY06 Progress Report … FY02–FY05 …

… experiment in the Sierra Nevada mountain range since 2014 to learn more about the microbial responses and feedbacks … since the collaborative science program was formed in 2014 by the Office of Biological and Environmental Research …

… sites: Microbes: IMG (citation: https://doi.org/10.1093/nar/gkaa939 ) Metagenomes and Single Cells: IMG/M   (citation: … Phytozome (citation: https://doi.org/10.1093/nar/gkr944 ) The following tools are available for functional …