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The Power of One, Amplified
… A few years ago, a candidate bacterial lineage was identified from microbial and metagenome sequence data sets … was then used to produce a paired amplicon dataset (a.k.a. 16S rRNA gene sequencing), a shotgun metagenome, and a … User Facility, is supported under Contract No. DE-AC02-05CH11231. This work was supported in part by a Natural Sciences …
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From Stress to Growth: The adaptive mechanisms of algae in changing Arctic waters
… rapid environmental changes. Researchers were able to identify specific genes that allow the algae to adapt to sudden … dead cells recorded. Samples were taken again at two, six, 12 and 24 hours. By the 24-hour mark, these algae showed … U.S. Department of Energy under Contract No. DE-AC02-05CH11231. …
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DAP-seq Drives Robust Mapping of Gene Regulation
… bioenergy and bioengineering applications. “We worked with 10 species ranging from grasses to broccoli and trees like … Advancing gene regulation mapping Previous methods to identify where transcription factors (TFs) — the proteins that … Complementing this, researchers have profiled over 160,000 high-quality single-nuclei transcriptomes, enabling …
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JGI Scientists Pen Genome Watch Articles
… by its genomes and metagenome , rather than just by 16S ribosomal RNA gene censuses.  Emiley Eloe-Fadrosh ,  … and computational analysis that enable systems-based scientific approaches to these challenges.  DOE’s Office of …
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New Vision, New Mission, New Plan: The JGI’s Next Five Years
… and environmental remediation—all priorities identified by the DOE Office of Biological and Environmental … range of new tools and capabilities to support their scientific investigations of biological and environmental … plan goes beyond outlining a broad vision and multiple scientific, technological, and operational themes and objectives …
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Building Wet and Dry Lab Skills
… the plants and extracting DNA. They also analyzed 16s amplicon data to determine microbiome composition. This …
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Expanding Virophage Diversity
… the number of known high quality virophage genome sequences 10-fold through computational approaches. By mining more … the virophage marker gene MCP, they were able to identify 44,221 total virophage partial sequences, including … the gene counts have similarly gone up from 13-25 to 12-39. “Overall,” the team concluded, “we provide a global …
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DOE JGI Interns Share Their Summer Experiences
… This summer, the DOE JGI hosted 10 interns who came from a wide range of backgrounds. Many …
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Ancient Algae Reveal Secrets of Plant Evolution
… ancestry of these algae and land plants. Researchers identified key genes associated with stress responses and … Foundation grant 440231723 (VR 132/4-1) to J.d.V., TH417/12-1 to G.T. and F.R., and 440540015 (BU 2301/6-1) to H.B. … to Land: Plant Evolution to Change’ (SPP 2237), and grant 410739858 in the frame of the project CharMod to K.v.S., as …
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Crysten Blaby-Haas
… for Science, 2020 NIH NRSA Postdoctoral Fellowship (F32), 2012 – 2015 Bright Futures Academic Scholarship, 2002 – 2006 … extremophilic algae reveals iron economy management. PNAS  120(30):e2305495120. Pasquini M, Grosjean N, Hixson KK, … aminopeptidase. Cell Reps 39 (7), 110834. (Subject of News and Views Article in Nature 608, 38-39 (2022) and …
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Mining Public Databases Yields Largest Map of Fungal Mitochondrial Genomes to Date
… energy metabolism. The Science Researchers produced nearly 10,000 new annotations of fungal mitochondrial genomes — the … largest dataset of its kind ever assembled. The team identified 15 core genes shared across all fungi, and found that … size and structure varied enormously, ranging from just 12 kilobases in Rozella allomycis , an early-diverging …
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