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High Throughput Phosphoglycolate Phosphatase Activity Assay Using Crude Leaf Extract and Recombinant Enzyme to Determine Kinetic Parameters Km and Vmax Using a Microplate Reader
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Model Fern Reveals Insight into DNA Thievery in Ferns
… — making it difficult to grasp a deeper understanding of fundamental aspects of fern biology and, more broadly, … to protect themselves from environmental stresses, ferns’ secondary metabolites and associated genes are great resources for …
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Help from Citrus’ Bitter, Hardier Cousin
The chromosome-scale genome sequence of trifoliate orange yields clues to disease-resistance that … from greening disease. You’ve probably never heard of trifoliate orange. Otherwise known as  Poncirus … may be a plant of bitter fruit, yet sweet are its genetic secrets. This work builds upon JGI-supported research led by …
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Doubling Down on Known Protein Families
… online Oct. 11, 2023 in  Nature highlights the vast array of functional diversity of microbes through a novel approach to better understand … “So, that diversity is definitely holding a lot of new secrets in terms of functional diversity as well.”  …
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FICUS
… for researchers to request the capabilities and expertise of multiple DOE National User Facilities in one research … Successful applications will focus on high-risk/high-payoff proposals within the focused topic areas that can be … such as DNA-SIP, Protein SIP, Flux Balance analysis with metabolites, Isotope Ratio Mass Spectrometry, NanoSIMS, and …
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The Power of One, Amplified
A preview of how large-scale single cell genomics complements … studies. The Science Researchers demonstrated the value of conducting large-scale single cell genomics by collecting … Such techniques take advantage of bioinformatics tools for  extracting snippets of microbial genomes directly from …
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A Revamped Amplification Method that Enables Nearly Complete Single-Cell Genomes
… from single cells. The Science Studying the  genomes of microorganisms is incredibly useful for understanding the microbiome of the earth, but most species do not grow easily in the … sequencing and single-cell sequencing. For years, extracting single cells and then amplifying their DNA for …
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Katherine Louie
… efforts to expand the metabolomics capabilities and product offerings for JGI users.  Using cutting-edge mass … techniques, the platform delivers rich metabolite profiles from diverse biological and environmental samples in support of user science.  Capabilities offered by the platform are a …
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JGI@25: Tracking & Subduing the Plague of California’s Oak Woodlands
In honor of the JGI's 25th anniversary in 2022, we have revisited a number of notable achievements that showcase our collaborations and … of SOD, the debilitated trees become susceptible to other secondary assaults, such as those posed by bark-boring …
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Supercharging SIP in the Fungal Hyphosphere
The Science Researchers have successfully automated aspects of a commonly-used process for studying microbial … with results published in the  November 2022 issue of Microbiome . High-throughput stable isotope probing (SIP) … up the pace at which researchers can extract the bioenergy secrets they hold. HT-SIP is a capability available to …
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JGI@25: Studying Sorghum’s Survival Skills
In honor of the JGI's 25th anniversary in 2022, we have revisited a number of notable achievements that showcase our collaborations and … sorghum’s hardiness, so understanding sorghum’s survival secrets could change not only how we use and grow sorghum, …
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Streamlining Regulon Identification in Bacteria
… with their target genes. The Science Regulons are a group of genes that can be turned on or off by the same regulatory protein. Researchers demonstrated … scientist Hiroshi Otani. Both are members of the JGI’s Secondary Metabolite Science Program, which Mouncey leads. …
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