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… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … cousins. So we both went to Scripps [Institution of Oceanography in San Diego] together. And I worked for Brad … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … and coastal. But we’ve kind of shied away from the ocean. Microbes. We’ve done those in the past, but the DOE …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … think the terms we should be using for what we do are, and why there and why it’s important to make those distinctions. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … there you go. [laughs] ALISON TAKEMURA: All the more reason why it could have been inspired by Super Smash Brothers. DAN …

… study of microbial communities in the northeastern subarctic Pacific Ocean. The Impact The microbial communities in the … samples. Genomic DNA extracted from the water samples were sequenced by the U.S. Department of Energy (DOE) Joint …

… polewards, they’d oust resident tiny algae. The cold polar oceans give rise to some of the largest food webs on Earth. … “A lot of our food comes from the North Atlantic, North Pacific and South Pacific fisheries, because of eukaryotic … than previously suspected. “That would cause significant consequences on the entire food web, and therefore ecosystem …

… you would think. Anything that could be swimming in the ocean right?  ALISON: Follow up question: Does the organ … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …