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… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … cousins. So we both went to Scripps [Institution of Oceanography in San Diego] together. And I worked for Brad … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … and coastal. But we’ve kind of shied away from the ocean. Microbes. We’ve done those in the past, but the DOE …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … think the terms we should be using for what we do are, and why there and why it’s important to make those distinctions. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … there you go. [laughs] ALISON TAKEMURA: All the more reason why it could have been inspired by Super Smash Brothers. DAN …

… polewards, they’d oust resident tiny algae. The cold polar oceans give rise to some of the largest food webs on Earth. … “A lot of our food comes from the North Atlantic, North Pacific and South Pacific fisheries, because of eukaryotic … than previously suspected. “That would cause significant consequences on the entire food web, and therefore ecosystem …

… you would think. Anything that could be swimming in the ocean right?  ALISON: Follow up question: Does the organ … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

The bacterium SAR324 is unusually cosmopolitan. In the ocean’s North Pacific Subtropical Gyre, microbes tend to stay localized at … of SAR324 cells and their gene expression, the team then sequenced community genomes and expressed gene transcripts …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … or you know, you’ve run into challenges that demonstrate why this is so important. BEN:  Yeah. So I wouldn’t call it …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … sampling which we would look at different particles in the ocean and look at the micro organisms on those particles, as …