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… grade list of goals in life: on that list are being a marine biologist and being a chemist, and it’s actually … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … and so we were able to actually not dive to collect the marine tunicates, which was nice because it’s also cold. And … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the … are actually are constructing a BGC, a Biosynthetic Gene Cluster. So we do have enough data, and if you follow them, …

… using single cell approaches. This includes linking strain-level genetic variation to ecological niche … laboratory isolation and genome sequencing of underexplored bacterial and archaeal lineages that are important for … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from …

… cell types and associated organisms, including fungal and bacterial symbionts, detrimental microbial pathogens, and … viruses. Resident viruses can be found in a myriad of bacterial strains, whereby they can modulate host metabolism, gene …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … come from all kinds of disparate environments. There’s some marine. There’s some soil samples. There’s human gut …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … the community so far. But being here and then being at the Marine Natural Products meeting in Ventura where I got to …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … U.S. Culture Collection Network (USCCN) to nominate their strains for sequencing, as long as they have a scientific … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… with only a small portion of the biosynthetic gene clusters (BGCs) being expressed. The program works to … based systems to express BGCs that alleviate the constraints of cells and allow for substrates to be directed … computational analyses of BGC size and complexity, BGC sequence composition, metabolic similarity & compatibility, …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … clone 170/NA DNA Synthesis Constructs >10kb in size Gene clusters or pathways totaling more than 10kb in length …

… working with him.  We talk about his father, the legendary marine natural products chemist, Richard Moore, and get into … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …