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… Awesome. Yeah, I’ve heard a lot of good things about Ben. Nice to meet you, Ben. BEN: Hey, yeah! Great, I’m very happy … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … put into antiSMASH after downloading it was isolated from Lake Washington. So it’s really interesting that it was …

… If you have concerns about shipping your DNA/RNA on dry ice, Gentegra products may be appropriate alternatives. See … The JGI asks that you please use the appropriate JGI Auspice Statement to acknowledge the work done by the JGI. More … I request a culture/slant/DNA of an organism that has been sequenced at the JGI? …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … to be big pieces somewhere, right? The National Park Service has a great writeup on the kind of gold mining Dan is … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… through its data portals. Supported by the DOE Office of Science's Biological and Environmental Research … which manages Berkeley Lab, leased laboratory and office space in a light industrial park in Walnut Creek, … in doing so enabled the JGI to be the first to publish the sequence analysis of the target chromosomes 5, 16, and 19, …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … to less than 5kb in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs …

… I must’ve been around about 18. And I went to Marcel’s office. And he’s incredibly enthusiastic and gave me a bunch of … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … MIBiG, and newer efforts, like BiG-scape and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… Our research focuses on the assembly of metagenomic sequence data into microbial genomes, which is of … Profile HMM search across all IMG metagenomes, capturing sequence diversity for proteins of interest. … better understanding of complex ecosystems within hard to sequence and assemble samples, such as those from soil and …

… and they’re very beautiful. It’s something that I’d noticed as a kid just being out in the ocean, you can see these … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… NERSC ) is to accelerate scientific discovery at the DOE Office of Science through high performance computing (HPC) and … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on … project are coordinated through JGI’s Project Management Office.  At the completion of a project, the JGI makes the …

… the user project (such as chemicals, basic lab supplies, office supplies) is supported by the Foundry. If a user’s … We recommend using a full-service courier (more information and some recommended … and will continue to keep the samples frozen, or load dry ice, or whatever is needed. A potential benefit of this …