Search

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … and where we are today. After that, we have lots of great conversations that we’ve already recorded with some … tell.  ALISON: It could be bacterial blankets, or coats, or water bottles, in a metaphor…. DAN: Or because they’re …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … that just melts in your hands, that’s not going to be great to work with. If you have an icy, hard packed snowball … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …

… one of the best things that we do is work with this really great group of biologists that know what they’re doing much … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … scientists who also do genome mining. We’ve got a great, fun bunch of little shows lined up, and I’m really … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene …

… of challenges that usually lead to surprises and really great discoveries! And Eric’s done an awesome job, in that … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… working on it because I really do want you all to hear this great conversation with Aaron Puri. He is an Assistant … of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … physical separation of a biologically relevant unit from a microbial community, such as a microcolony, microbial …

… DAN UDWARY: Hey, everyone. I’ve got a great episode for you here today. We got the opportunity to … learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… Cooperative Biodiversity Groups, and it’s this great program funded by the National Institutes of Health … Apple Podcasts or wherever you’re getting this and tell me why you’re listening. DAN: But, now, here’s Natural Prodcast … And that’s been followed by developments in pulse sequences and improvements in the ways in which you acquire …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … Two fanatic PhD students. Alison Takemura: It sounds great. Yeah, it sounds like it was really needed at that …

… after listening to this, you think you actually do have a great project that would awesome for a CSP, or if you’re a … at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …