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… sequencing projects, including 10,000 Actinomycetota strains and 10,000 type strains. These efforts will provide … resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes …

… learned a lot so much history from this conversation about why the ocean was basically unexplored until the 70s, and … the plate. Look at this, nothing grew. Three days, five days, nothing’s growing. Unfortunately, you have to … forward to seeing the data when it finally gets off the sequencers. The pandemic has obviously slowed JGI down a …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … is, where do you think your lab is going over the next say five or 10 years? Sounds like function is a big part of …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …

Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the … a time course. And you might have done the extraction at five days, seven days, 10 days. And suddenly, you’re then …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … there you go. [laughs] ALISON TAKEMURA: All the more reason why it could have been inspired by Super Smash Brothers. DAN …

… already had a fermentation process with already engineered strains, but this was to further build on those to try and … to some people more about down the road. Email Nigel! He’ll sequence whatever you want!  [LAUGHTER]  NIGEL MOUNCEY: We … of the publications committee for the last, what is it now, five years, working through two journal contracts for the …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … what we’re trying to do right now is develop the chassis strains that then we can port things into as well. DAN: Give …

… embargo starting at construct or strain delivery.  Detailed sequence information for constructs are made publicly …