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… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … I have been in – working in secondary metabolism for almost 20 years now. Which when I did the math, that was a surprise …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … has to do with the mechanism. And so these enzymes use O2, and one of those oxygen atoms gets incorporated into our …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … in the marine ecosystems and marine actinomycetes and cyanobacteria. And we thought we could also– let’s see what …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … recent example of some of her work on molluscs and their cyanobacterial feeding preferences A 10″ Simple Rules” …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean … had just not  been released yet. And there was also some transcriptome data. And my first task during that project …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes … automatically identified from genomes, metagenomes, and metatranscriptomes. It provides rich data such as inferred host …

… to the second episode of the Natural Prodcast. This is part 2 of our “primer” episode, so if you ended up here somehow … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … the minimum is 100 kb, and for metabolomics, at least 22 samples are required. If your proposed request falls … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… we discussed in the primer podcasts ( part 1 here , part 2 here , and part 3 here ), which you should be able to find … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

Natural Products conference, which took place last January 2020 in San Diego. San Diego, of course, is the “home turf” … then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against …