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… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …

… that will help solve energy and environmental challenges. Chlamydomonas is a single-celled alga with a unique place in … sustainable biofuel production. The JGI published a partial sequence of Chlamydomonas reinhardtii in 2003; then in 2007 …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … I’ve seen you give a few talks on that. Can you tell us why cone snails are so cool? ERIC:  So cone snails are … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… about the basics of genome mining, which is using DNA sequence to identify and interpret biosynthetic, secondary … data sets and seeking to interpret function from BGCs. It’s a long, wide ranging conversation and I’m so … biological perspective, like what was this bacterium, and why did your group want to study it? Marnix Medema: You mean …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … cycling and bioproduct applications.  The team has three subgroups: Synthetic Biology Lab : a user science group … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

… resequencing, RNA sequencing and epigenomics. Expansion of sequence space:  The JGI generates reference genomes from … of microbial communities based on their metagenome sequence, in the context of reference isolate genomes … automatically identified from genomes, metagenomes, and metatranscriptomes. It provides rich data such as inferred host …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … – how bacteria or other organisms communicate and how and why secondary metabolites are being produced in the … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … this and that. They’re doing all this 16S and I said, “Why don’t you just include the ITS too? It’s so easy to add …

… then genomics happened. Dan: Yeah, I did my first genome sequence with you. Brad: That’s right,  Dan: JGI did it in … working with you, Dan, on this one, and we said, “Well, why don’t we have you know, the bioinformaticians go against … get made fun of by my now-wife, because she was wondering why it is that we needed to produce more toxins in the lab …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … of analysis pipelines, be it for, again, metagenome, metatranscriptomes for SNP analysis if somebody has a … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …