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… communities contribute to critical processes such as carbon cycling, nutrient cycling, and energy flow. One of … comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … For example, lipids– doesn’t matter if it has one extra carbon or two or one less, at the end of the day, it’s … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… metabolic flexibility? … Microbial contributions to soil carbon storage during simulated range shifts of plant-fungal … of simplified soil communities with high and low carbon use efficiency across differing moisture treatments … …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … and work within the DOE emission space of bioenergy, carbon cycling, biogeochemistry, bioproducts. So we cannot … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … metabolites evolve– who makes them, how do they spread, why do they spread in a certain way from one bacteria to … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have … But… DAN: Yeah, there are a lot of ways to put together carbon molecules. Chemistry is pretty limitless. All right, …

LanzaTech, a Skokie, Illinois-based company that recycles carbon emissions to make fuels and chemicals, improving air …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to … between chemistry and sequence to get anywhere. Like that’s why I say the more you know, the more you know because it …

… capabilities support researchers exploring how to convert sequence into functional assessments. This is done by … design tool  … BOOST provides a suite of tools to automate sequence design for assembly by Gibson, Yeast recombination …

And at the time, it was really exciting when people would sequence [DNA]. The genome was– well, that hadn’t really … polyketide synthase and then P3. We still don’t know why 20 years later. JACKIE WINTER: It’s pretty amazing, … And that’s what I really wanted to do. And that’s why I came back to academia. And I set that up. And we …