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… such effort is targeted at environmental cells that are from candidate phyla, major branches in the phylogenetic … of microbial metabolism towards a minority of cultivated bacteria, still persists to date. Research Team    … … is also reflected in the strongly biased representation of sequenced genomes in the public domain, the bulk of which …

… biology classes in undergrad, but my PhD was an actinobacteria. And I tell all my students this now, but when I … Duncan: Yeah. I love actinomycetes. Dan Udwary: Tell us why. I mean, I know, but I think– Alison Takemura: I want to … of almost finished characterizing them. We’ve got genome sequences, which are just incredible. We’ve looked at the …

… cell types and associated organisms, including fungal and bacterial symbionts, detrimental microbial pathogens, and … This is a dynamic and complex ecosystem that includes bacteria, fungi, viruses. Resident viruses can be found in a myriad of bacterial strains, whereby they can modulate host …

… about something I can kind of see like the squid and the bacteria and how the chemistry is going back and forth … does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … origin is around 30,000 from fungal, and a 40,000 from bacteria. Okay? And among the 40,000 from bacteria, there’s …

… about several different pieces of her work, including bacterial quorum sensing and its effects on regulation of … The genes are pretty much 100% identical. We don't know why one is expressed over the other. As far as whether we're … usually serve as dimers, and then they will bind to certain sequences of DNA. And when they bind to those certain …

… through the stories I told you about geosmin, which is a bacterial sacred metabolite, and ergot alkaloids come from … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… been dominated for … well, as long as I can remember, by bacterial research.  So it was great to hear her passion and … out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk …

QC Protocol DNA Extraction 101 : tips, kits, and protocols (from PacBio) Guidance for sample collection and storage (from PacBio) Best practices for High Molecular Weight DNA … pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … on identifying and characterizing haloperoxidases from bacteria. And with Christian, I started getting a little bit … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… we have our conversation with Professor Alison Narayan, from the University of Michigan. I hope she’ll accept this … enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh …

… colleagues. Aaron is doing some cool stuff with cool bacteria that I’m fascinated to learn more about. He works … of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. …