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… in their natural environments. This work encompasses bacteria, archaea, and micro-eukaryoes, along with their … lineages. Charting viral ‘dark matter’:   Viruses are abundant in the biosphere and influence microbial dynamics, … comprising the largest public collection of plasmid sequences identified from genomes, SAGs, MAGs, metagenomes …

… And a lot of us moved on to work in simpler systems, like bacteria, where we figure we can make more progress faster, … you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… and comparative analysis of thousands of archaeal and bacterial type strains ( GEBA-type strains project ), the … al. (2017) Protein structure determination using metagenome sequence data. Science   355(6322):294-298 Paez-Espino D. et al. (2017) IMG/VR: a database of cultured and uncultured DNA Viruses and retroviruses.  Nucleic Acids Res. …

… out introns and dealing with, you know, weirdness in the sequence. You know, some clusters are not clustered…   … it so easily with fungi. So…   NANCY: Yeah, I don’t know why. For example, with that – actually there’s a good talk … Is that the case for fungi?   NANCY: I think a lot of labs yet are doing that now, because it’s gotten so, so cheap to …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… cell types and associated organisms, including fungal and bacterial symbionts, detrimental microbial pathogens, and … This is a dynamic and complex ecosystem that includes bacteria, fungi, viruses. Resident viruses can be found in a myriad of bacterial strains, whereby they can modulate host …

… at genomes and seeing where the really hard things to sequence were, and wondering what those … what those were … functional was these secondary metabolite pathways. So why was that? What is it about secondary metabolite pathways … We’re not perfect at identifying what these pathways are yet. So there’s still a lot to be discovered. And even the …

… DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have … ALISON: It’s wild. It kind of looks like a sock puppet of a Yeti with a large purple lollipop attached to its mouth. …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … want to try to get into or maybe haven’t had the chance to yet? I mean, you’ve been there a few years now, but it … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… what he just said here. It was actually the drug company Wyeth that introduced enediynes to the market in 2001 with a … the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … would install that moiety? Because maybe you can’t do it yet synthetically, because you have reactive groups around …