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… the only way a primary metabolite or a primary metabolic pathway is good for anything, it is if the final product is … the active site of an enzyme. So there is no promiscuity, generally speaking, in primary metabolism. Nevertheless, … that single changes can lead to a particular high activity towards a particular target. That’s why an antibiotic has to …

ALISON TAKEMURA: I know that it’s looking in the genome for genetic elements of interest, you know, something like … Where did you see that research or those results heading? Toward an application, toward just better understanding … amino acid out of solution and stick another amino acid or multiple other amino acids onto it to create a small …

… identify and interpret biosynthetic, secondary metabolism pathways. And today, we get to present our conversation with … and BiG-SLiCE, and now the BiG-FAM database of biosynthetic gene cluster families. And he tells us about forthcoming … encoded by them. Well, they had come out over actually multiple decades of time. But still to be able to use those …

… genomics existed. This is before we’re even thinking of genes and connecting genes to molecules, which is something … , but circular. 10% of the genome encoded natural product pathways. Dan: Yeah, that’s kind of a trend now. But at the … of environmental health that we’re now working on. And this multi investigator new program that NOAA has funded to …

… right, title, and interest to any intellectual property generated under the agreement. … CRADAs enable research … Area to quantify atmospheric gas mitigation properties of multiple rice plant growth promotion technologies using … single-step genomic integration and prototyping of complex pathways. The team will be working with LanzaTech to rapidly …

… and actually get to the DNA sequence – sequences for the genes that code for the proteins that produce secondary … you’re going to find another 20 or 30 secondary metabolism pathways that you didn’t even know existed. And so, that … has the opportunity to, you know, use that research towards secondary metabolism aims. And we need new people – …

… … DAN UDWARY: You’re listening to the Department of Energy Joint Genome Institute’s … of it. So it wasn’t only chemistry or only biochemistry or genetics. It was also ecology. It was also evolution. It was … There’s gene fusion going on, and then I could see how the pathway gets streamlined and becomes more effective. That’s …

… and he has a project with the JGI to synthesize the genes for and explore the chemistry of quorum sensing … usually very beneficial to the field. It’s very, very, very multidisciplinary, is the usual term for that. So yeah. … to go. What’s the bigger– is there a bigger picture here towards this research? And what do you want to get out of …

… its effects on regulation of natural product biosynthetic pathways, which led her to a successfully funded JGI CSP … oftentimes will bind to receptors within biosynthetic gene clusters. Specifically, these are Tet repressors that … And so that thioesterase at the end of this large multimodular protein allows for release from the DNA, either …

… years before joining Warp Drive. And what kind of pushed me towards the secondary metabolism side of things was looking … breaking our methods was these damn secondary metabolite pathways. So that’s kind of how I fell down the rabbit hole. … that can’t live without their host. They have no extra genes hanging around, they still dedicate 20-30% of their …

… … DAN: You’re listening to the US Department of Energy Joint Genome Institute’s … what we’re calling a Primer. We wanted to try and give a general audience some of the basics of natural products … ALISON: Okay. We’re looking at a schematic of different pathways in a microbial cell, it looks like. It could be a …