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… work. And that it involves some of these crazy genes, some of these enormous genes that encode these very large enzymes. And I was like, … end up being post-translationally modified into ribosomally synthesized peptide natural products. And they built a few …

… that’s not too hard. But then you need to test it. Some testing might be hard, depending on how you’re doing the testing. If you have a successful test, then you need to go … and actually get to the DNA sequence – sequences for the genes that code for the proteins that produce secondary …

… for the generation of sequence or metabolomic data, DNA synthesized, and any other contributions, such as assembly, …

… this interview are the RiPPs, which stands for “Ribosomally synthesized and Post-translationally modified Peptides.” … really need to know how they’re made. You need to bring the genes into the lab, and you need to manipulate them in the … are classed as “RiPPs”, which stands for “Ribosomally synthesized and Post-translationally modified Peptides.” …

… organisms actually have quite a few antibiotic-resistant genes in there. And so we’re trying to use those as … on that we sequenced the genome. And it has 17 resistant genes on a plasmid that’s actually– it’s pretty scary what … Every tool will tell you something different about the genes or homology to something. There’s a lot– every BGC is …

… the regulation off, rather than negatively get rid of the genes from their genome. FRANCISCO BARONA-GOMEZ: Exactly … gene cluster starts to merge and expand and accept new genes coming in and out. So, if you start making the … find these definitions useful and we can really start testing and organizing things beyond artificial names. …

… really, really falls rapidly. But we eventually– after testing all these different zones, we eventually settled in … mining-based approaches are that other than resistance genes, you have no way of knowing of whether or not a … about 20 analogs at this point. And Casey and Nikolaou synthesized the everninomicin at Scripps, I guess, 22 years …