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… mining company, back when DNA sequencing of natural product pathways was hard and expensive to do. As I said, Brian is … So we started to see this potential of looking for compounds based on these gene clusters. And that was my job … colors. I mean, where else in nature– and they’re not fungal. Where else nature can you go see colonies of …

… that consists of coordinated hands-on experiments across fungal biology teaching labs. The purpose of the program is … and data analyses compile original data for improving fungal genomic resources empower students through isolation and genome sequencing of new fungal species.  Students collect fungi, sterilize tissue, …

… Yeah, so any metabolite, produced by bacterial or fungal metabolism by microbes, will have a functional role. … For example, lipids– doesn’t matter if it has one extra carbon or two or one less, at the end of the day, it’s … bit more intuition about how you go from the expansion to comparing to the database. Is it like, let’s say I’m drawing …

… Microbial contributions to soil carbon storage during simulated range shifts of plant-fungal symbionts … of simplified soil communities with high and low carbon use efficiency across differing moisture treatments …

… nutrient acquisition and communication. Studying how these compounds are biosynthesized and their roles in the … produced by bacteria, fungi, algae and plants. These compounds are key to organisms' interactions with their … gene clusters (BGCs) being expressed. The program works to uncover these hidden biochemical resources by exploring the …

… identify and interpret biosynthetic, secondary metabolism pathways. And today, we get to present our conversation with … that, OK, I find 25 gene clusters, there must be 25 new compounds. Or everything that has similarity to a gene … are protecting the plants against pathogens. So usually fungal or oomycete pathogens. In this case they worked on a …

… where I was a graduate student. Aflatoxin, of course, is a fungal natural product which is a liver toxin when humans … people looking at sponge chemistry and looking for active compounds and pharmaceuticals. ALISON:  Okay, and he was … are also many other stories, waiting for good scientists to uncover. DAN:  So you’ve also done a lot of work on cone …

… that I’m working with about now. I made a tree of different compounds, and I’d looked at it from an evolutionary … colleague who’s working on structural biology of these compounds. And they saw the tree, and I explained to them … seeing that. And I’m always just looking from the genes and pathways and what’s happening there. And he really saw this …

… it has a really interesting biosynthetic pathway because fungal genes are cool. And if you like fungal genes, you … oxidative conversions and do some really complicated carbon skeleton rearrangements, chemistry that we probably … having a baseball glove or some kind of glove, and you’re comparing different gloves. And then if you get enough …

… me really interested in microbial gene regulation and some pathways. And I thought I’d take an academic job then, but I … which– you’ve got this very sugary environment. It’s a compound that clearly kills things that would otherwise … If you’re interested in partnering with us to test compounds that we’re isolating, please let us know.  DAN: …

… question, are these plants and animals making chemical compounds in a way that’s similar or analogous to what’s … phylogenetic tools measuring genes, the 16S gene, and comparing it with everything that’s known. So Salinispora , … time, we found another molecule. This time it was from a fungal strain collected from sediments in the ocean. No, …

… DNA Synthesis Constructs >10kb in size Gene clusters or pathways totaling more than 10kb in length assembled … to determine coverage of variants depends on project Fungal Minimal Draft Lower coverage short-read whole genome … sequencing Assembly, annotation ( Mycocosm ) 256/404 Fungal Standard Draft Whole genome shotgun sequencing using …