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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … so far no species or no strains known that really grow in freshwater. But they’re actually pretty orange, and they … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … of the field. Then, in the third episode, we’ll discuss the modern history of  natural products and where we are today. … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … and it’s equivalent to translating all these books into a modern language that everybody can read. So now, if you …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… on, in the next episode you’ll hear us talk about the modern history of natural products and the state of the … DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… at JGI start with sequencing, but where else did those sequences go? Like, what are the other scientific tools we … that describe the work. In general. Scientific merit and why it’s important. The DOE mission is one of these … are marine related, and so we can do some projects that are freshwater and coastal. But we’ve kind of shied away from …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … DAN UDWARY: That’s weird. I hadn’t heard that before. Why is that? Why would that be the case? ALISON NARAYAN: …