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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … now that I think is going to look totally different in five years than it does right now. So look out. DAN UDWARY: …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … because this also reflects over the past, probably, say, five or six decades, the merger of many pharmaceutical …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… stories about natural products, so you can get a feel for why I think they’re so important, and we’ll start to explain … more about some of the background and sort of the reasons why we want to do this. And also to provide a little more … of the fun things that I hope that I can express today is why secondary metabolism is interesting and why it is that …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … systems. So, in those cases, it’s been, in the last five years, obvious that these are specialized metabolites. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … there you go. [laughs] ALISON TAKEMURA: All the more reason why it could have been inspired by Super Smash Brothers. DAN …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … metabolites evolve– who makes them, how do they spread, why do they spread in a certain way from one bacteria to … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …