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… expression and functional exploration.  The developed strains represent an additional phase of user-accessible … into a taxonomically diverse suite of microbial chassis strains for elevated opportunity for expression. Research …

When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … because this also reflects over the past, probably, say, five or six decades, the merger of many pharmaceutical …

DAN: Alright, so I think we’ve covered a little bit about why they’re important. But one of the things I did want to … I studied in the past were a group of bacteria called the Frankia . And so the Frankia are bacteria that live in the root nodules of plants. And these guys were sequenced, and you can see that different Frankia have …

… capabilities and products . A minimum request of 1 Tb of sequence data is required to qualify for BERSS. For DNA … U.S. Culture Collection Network (USCCN) to nominate their strains for sequencing, as long as they have a scientific … and personnel resources for genomic research and sequence-based science.  Projects include de novo generation …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … in length, cloned into vector of choice Glycerol stock of sequence verified clone 170/NA DNA Synthesis Constructs …

… is like, what’s your origin story in natural products? Why are you doing this? JACLYN WINTER: I’ll kind of go back … have an E. coli strain that we’ve been working on that we sequenced the genome. And it has 17 resistant genes on a … , and you taught everybody how to look at the different Frankia genomes. And we had three genomes. We did …

… does some really exciting work there. And I thought, well, why don’t we try to go to Alaska and see what we can find … advisor] Brad Moore – who we talked to in, what, episode five – that’s one of his things was for a long time and I … with any kind of gene cluster identification, doing it with sequence alone you have to have some kind of a template to …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … they’re obligate marine. There’s so far no species or no strains known that really grow in freshwater. But they’re … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… enzymes are, right? ALISON NARAYAN: Yes, and so that’s why I don’t like that sentence. I think that sometimes, we … association that might help? Like, if you have an unknown sequence or new flavin monooxygenases, you could say, oh … now that I think is going to look totally different in five years than it does right now. So look out. DAN UDWARY: …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … systems. So, in those cases, it’s been, in the last five years, obvious that these are specialized metabolites. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …