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When submitting sequences to the JGI DNA Synthesis program, please include the following items: … Sequence files for all constructs should be emailed to  … several easy-to-use open source tools for converting DNA sequence files into well-formed GenBank files. ApE is an …

… sequencing products below. In addition, JGI will submit raw sequence data to  SRA at NCBI once the standard analysis is … in length, cloned into vector of choice Glycerol stock of sequence verified clone 102/NA DNA Synthesis Constructs … structural rearrangements, alignment files 384/454 Fungal Transcriptome RNA for expression profiling and genome …

… there are any kind of dated references to the news, that’s why. But it was a really fun conversation. And I think … later. And she happened to work with nature products from cyanobacteria. So that’s how I started. I love the lab, I … it would work, but we actually found fosmids. And we sequenced the whole fosmid at the time with Sanger …

… you maybe explain what’s going on with their biology and why they’re so important to natural products? ERIC:  Sure. … we were working with tunicates, and tunicates living with cyanobacteria, and various other types of bacteria. So I got … turns a peptide, for example, from a disordered sequence into an antibiotic that kills bacteria through a …

… of their synthases so that we can start to use the DNA sequence to predict or just understand better the language … your interest in getting into natural products. DAN: Why are you here at SIMB? AARON PURI: Yeah. Thanks Jackie. … expression hosts like maybe in many fields now like in cyanobacteria or even [ Streptomyces ] coelicolor you’ll see …

… pipelines.  You will receive automated emails as raw sequence data becomes available, and as analysis is …

… least, talk to us, first about my definitions of things and why I am wrong, and also talk about some of the great … think the terms we should be using for what we do are, and why there and why it’s important to make those distinctions. … to approach it is more related to what JGI does, which is sequence genomes. And we just published last year in the …

… more than 50,000 genomes that we derived from meta-genome sequences. As always, you’ll find transcripts and show notes … for us– what organisms people use for genome mining, why it’s called genome mining, how the biosynthetic gene … there you go. [laughs] ALISON TAKEMURA: All the more reason why it could have been inspired by Super Smash Brothers. DAN …

… … For sequencing projects, once work is under way, raw sequence data is released to NCBI’s Sequence Read Archive on a regular basis, in accordance with …

… the paradigm of how to discover natural products. So, why I’m very excited to be working with Dan, you, with the … develop enabling technologies. ie how to translate the ATGC sequence into discrete small molecules. So, Dan, I’m very … or you know, you’ve run into challenges that demonstrate why this is so important. BEN:  Yeah. So I wouldn’t call it …