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… Research in the Woyke laboratory focuses on the utility of single-cell methods to access genetic material of uncultivated taxa … of interest. One such effort is targeted at environmental cells that are from candidate phyla, major branches in the … of which belong to only three phyla. The Woyke group uses single-cell sequencing as a means to begin filling this gap. …

… automation — and further advancing sequence applications, single cell genomics, DNA synthesis, and metabolomics … JGI operates highly scaled short- and long-read sequencing pipelines. This includes advanced sample management (quality … method allowing high-throughput processing of samples in 96-well plates on automated liquid handler …

… brief description of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … detailing the quantity & quality requirements for samples submitted to the DOE JGI. DNA Preparation Guidelines … or entered into a web interface prior to shipping samples to the JGI. PI sends Project Manager (PM) a list of …

… mechanisms involved in virus:host interaction and host cell take-over by viruses, and attempts to evaluate … Genomics group attempts to address this question in natural soil ecosystems by combining metagenomics with other omics … in microbiomes, these omics data must be complemented with single-cell resolution assays that can be targeted towards …

… technology in order to enhance the quality of single-cell genomes and directly link functional information with individual cells. Research Team    … quantitative Stable Isotope Probing (SIP) Metagenomics pipeline that utilizes internal analytical standards to …

… addressing key environmental challenges, such as improving soil health, carbon sequestration and bioeconomy … of bacteria and archaea in their natural environments using single cell approaches. This includes linking strain-level genetic … and improved draft microbial genomes the program offers single-cell sequencing, resequencing, RNA sequencing and …

… We use multi-omics (metagenomics, metatranscriptomics, single cell genomics and phylogenomics) and machine learning to … possess genomes and particle sizes that rival those of cellular organisms. Giant viruses challenge the traditional boundaries between viruses and cellular life, providing unique insights into viral …

… Plus: SynTrack and associated tools, which track samples through the JGI’s Synthetic Biology pipeline as well as provide process automation support   …

… percentile*) DNA Synthesis Constructs <5kb in size Single gene or multiple small genes assembled to less than … Assembly, annotation ( IMG/M ), mapping 273/273 Metagenome Cell Enrichments Obtained by physical separation of a … active groups of organisms within a community. Individual samples are fractionated using a density gradient, and each …

… the program supports foundational research related to soil nutrient cycling and health, terrestrial biogeochemical … from a wide range of biological and environmental samples — including microbes, fungi, plants and soils. Through a combination of experimental workflows and …

… come, though, too when you start talking about going after single gene clusters. BRIAN BACHMANN: That’s right. JACKIE … off through these areas. And you can do– with a teaspoon of soil from outside, you maybe have to do a 10,000-fold … mammalian systems. But it allows us to assay on a single cell level against cell lines and against primary cells …

… Accessing Resources at Multiple DOE User Facilities with a Single Proposal describes the resources available from the … at Arizona State University, which contains representative samples of the spatial, temporal, taxonomic (environmental, …