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The Sequencing Technologies Group applies cutting edge molecular capabilities and state-of-the-art next-generation sequencing to enable biological discovery by the JGI and external DOE users. Adopting the latest  sequencing technologies has allowed the JGI to increase …

… construct a bioenergy plant single-cell atlas comprising Sorghum, Switchgrass, and Brachypodium datasets. We recently …

… of the process needed to prepare and ship samples for sequencing by the JGI.  Additionally it includes … material is one of the greatest predictors of a successful sequencing project. It is imperative that users properly … nucleic acid quantification in our Illumina and PacBio sequencing workflows and provide guidance as to how to …

… that will help solve energy and environmental challenges. Sorghum stands out as a candidate feedstock for biofuels … JGI: 2009: JGI and collaborators use whole-genome shotgun sequencing to analyze the 730-megabase Sorghum bicolor (L.) … it with over 50 other diverse sorghum genomes. Deep sequencing and genetic linkage analysis improve the genome’s …

… and increasing demand for agricultural products.  Sorghum bicolor (L.) Moench is an African grass that … and poor soils. This is the first paper that describes sorghum’s response to drought, from a large-scale field … nearly 400 root and leaf samples and helped analyze the sequencing datasets. The results from the first year showed …

… any work can begin, including the submission of samples for sequencing, or the submission of sequencing data for synthesis. This agreement, required by … for Federal Agencies , DNA Synthesis Appendix  and DNA Sequencing Appendix are available for download.)  After your …

FY 2025 DOE Metrics Sequencing Quarter Total Bases (Trillions) Operating Hours …     Q4 2025 175     2,167     *Includes Illumina and PacBio sequencing platforms. **Operating Hours target is based on … even though an individual may have several projects/roles. Sequencing Quarter Total Bases (Trillions) Operating Hours …

The JGI has diverse capabilities in: DNA and RNA sequencing, Sample and library preparation, DNA synthesis … of standard analysis.  Raw data is provided for all sequencing products below. In addition, JGI will submit raw … have been  discontinued , including smRNA, bisulfite sequencing, and ChIP-seq. … Scientific Program Product …

PI and receive permission for their planned work.   … For sequencing or metabolomics projects: data are subject to a  … Policy … Data Management at the JGI … Policy Definitions … Sequencing and Metabolomics projects … DNA synthesis …

You might know sorghum as an edible grain. But there are some sorghum varieties, grown on marginal land with little water, … Mullet, a biologist at Texas A&M University, tells us how sorghum’s historical — and literal — roots could play a big …